Small-Angle X-Ray Scattering for the Study of Proteins in the Ubiquitin Pathway.
作者信息
Trempe Jean-François, Gehring Kalle
机构信息
Department of Pharmacology and Therapeutics, McGill University, Montréal, QC, Canada.
Department of Biochemistry, McGill University, Montréal, QC, Canada.
出版信息
Methods Mol Biol. 2018;1844:197-208. doi: 10.1007/978-1-4939-8706-1_13.
PMID:30242711
Abstract
Small-angle X-ray scattering (SAXS) is an invaluable complement to other biophysical methods used to interrogate the structure and dynamics of proteins. Here, we describe the standard experimental protocol used in our laboratory to analyze proteins in the ubiquitin pathway. The method addresses buffer selection, data collection using an in-house X-ray source, diagnostic tests to assess data quality, and computational approaches to interpret SAXS data.
摘要
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