Centre de Biochimie Structurale, INSERM, CNRS, Université de Montpellier, France.
Institute of Materials Structure Science, High Energy Accelerator Research Organization (KEK), 1-1 Oho, Tsukuba, Ibaraki 305-0801, Japan.
Biochim Biophys Acta Gen Subj. 2018 Feb;1862(2):253-274. doi: 10.1016/j.bbagen.2017.10.015. Epub 2017 Oct 26.
Clarification of solution structure and its modulation in proteins and protein complexes is crucially important to understand dynamical ordering in macromolecular systems. Small-angle x-ray scattering (SAXS) and small-angle neutron scattering (SANS) are among the most powerful techniques to derive structural information. Recent progress in sample preparation, instruments and software analysis is opening up a new era for small-angle scattering. In this review, recent progress and trends of SAXS and SANS are introduced from the point of view of instrumentation and analysis, touching on general features and standard methods of small-angle scattering. This article is part of a Special Issue entitled "Biophysical Exploration of Dynamical Ordering of Biomolecular Systems" edited by Dr. Koichi Kato.
阐明溶液结构及其在蛋白质和蛋白质复合物中的调节对于理解生物大分子系统中的动态有序性至关重要。小角度 X 射线散射（SAXS）和小角度中子散射（SANS）是获取结构信息最有力的技术之一。样品制备、仪器和软件分析的最新进展正在为小角度散射开辟一个新时代。在这篇综述中，从仪器和分析的角度介绍了 SAXS 和 SANS 的最新进展和趋势，涉及小角度散射的一般特征和标准方法。本文是由 Kato Koichi 博士编辑的特刊“生物分子系统动态有序性的生物物理研究”的一部分。
