Socha B M, Łada P, Szczepańska A A, Łupicka M, Korzekwa A J
Department of Reproductive Immunology and Pathology, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences (IARFR PAS), Tuwima Str. 10, 10-747, Olsztyn, Poland.
Veterinary Clinic 3VET, Ludowa Str. 78/3, 18-200 Wysokie Mazowieckie, Poland.
Theriogenology. 2018 Dec;122:74-83. doi: 10.1016/j.theriogenology.2018.09.013. Epub 2018 Sep 15.
Peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors of the nuclear receptor superfamily. The PPARs activity can be modified e.g. by arachidonic acid metabolites. Escherichia coli (E. coli) is one of the main infectious agent of endometritis in dairy cows. We hypothesized that PPAR expression profile change in the bovine endometrium under the influence of LPS or E. coli. In experiment 1, endometrial explants were obtained post mortem from heifers and incubated without (control) or with LPS for 12, 24, 48, 72 and 96 h. In experiment 2, heifers were intrauterine infused with 0.9% NaCl (control) or with E. coli suspension in 0.9% NaCl. Endometrial biopsies were performed before (0 h) and 12, 24, 48, 72, 96 h after the infusions. In experiment 1, the increase in protein expression was observed for PPARα 48 h, for PPARβ/δ 24, 72 and 96 h, whereas for PPARγ 12, 24 and 96 h after LPS treatment relative to the control groups. In experiment 2, the up-regulation in protein expression was observed for PPARα 48 and 72 h, for PPARβ/δ 72 and 96 h, for PPARγ1 and PPARγ2 12 and 96 h after the intrauterine infusion with E. coli suspension compared to the control group. Changes in mRNA and protein PPAR expression profile in endometrial explants under the exposure of LPS indicate participation of these nuclear receptors in signal transduction during stimulation with LPS. The patterns of mRNA and protein PPAR expression in endometrial bioptates suggest that during experimentally induced endometritis in vivo, PPARs role may be connected both with enhancement of inflammation as well restoring physiological conditions in uterus.
过氧化物酶体增殖物激活受体（PPARs）是核受体超家族的配体激活转录因子。PPARs的活性可被例如花生四烯酸代谢产物所改变。大肠杆菌（E. coli）是奶牛子宫内膜炎的主要病原体之一。我们推测在脂多糖（LPS）或大肠杆菌的影响下，牛子宫内膜中PPAR的表达谱会发生变化。在实验1中，从后备母牛死后获取子宫内膜外植体，在无（对照）或有LPS的情况下孵育12、24、48、72和96小时。在实验2中，给后备母牛子宫内注入0.9%氯化钠（对照）或0.9%氯化钠中的大肠杆菌悬液。在注入前（0小时）以及注入后12、24、48、72、96小时进行子宫内膜活检。在实验1中，相对于对照组，LPS处理后48小时PPARα的蛋白表达增加，24、72和96小时PPARβ/δ的蛋白表达增加，而12、24和96小时PPARγ的蛋白表达增加。在实验2中，与对照组相比，子宫内注入大肠杆菌悬液后48和72小时PPARα的蛋白表达上调，72和96小时PPARβ/δ的蛋白表达上调，12和96小时PPARγ1和PPARγ2的蛋白表达上调。LPS暴露下子宫内膜外植体中PPAR mRNA和蛋白表达谱的变化表明这些核受体参与了LPS刺激期间的信号转导。子宫内膜活检组织中PPAR mRNA和蛋白的表达模式表明，在体内实验性诱导的子宫内膜炎期间，PPARs的作用可能既与炎症增强有关，也与子宫生理状态的恢复有关。
