Garcia-Jimenez Waldo L, Villarreal-Ramos Bernardo, Grainger Duncan, Hewisnon R Glyn, Vordermeier Hans M, Salguero Francisco J
Department of Pathology and Infectious Diseases, School of Veterinary Medicine, University of Surrey, Guildford, Surrey, United Kingdom.
TB Research Group, Department of Bacteriology, Animal Health and Veterinary Laboratories Agency, AHVLA-Weybridge, New Haw, Addlestone, Surrey, United Kingdom.
Vet Immunol Immunopathol. 2018 Sep;203:52-56. doi: 10.1016/j.vetimm.2018.08.002. Epub 2018 Aug 13.
Bovine tuberculosis (bTB), mainly caused by Mycobacterium bovis (M. bovis), is a major economic disease of livestock worldwide. Vaccination is considered as a potentially sustainable adjunct to the current control strategy. Cattle vaccination with the live attenuated M. bovis bacillus Calmette-Guerin (BCG) confers variable protection; the reasons for this variability are not understood. Indoleamine 2, 3-dioxygenase (IDO), through the catalysis of tryptophan, is thought to have an immunoregulatory role in the immune response to Mycobacterium tuberculosis (M. tuberculosis). In this work, we used immunohistochemistry and digital image analysis to evaluate the presence of IDO in granulomas at different stages of development in cattle that had been BCG-vaccinated or not and then challenged with M. bovis. Our results show that the expression of IDO in granulomas from non-vaccinated M. bovis challenged animals is higher than in granulomas from BCG-vaccinated M. bovis challenged animals. Thus, it is possible that vaccination with BCG prevents the induction of what are thought to be host immunosuppressive pathways by M. bovis, which contribute to pathology during the disease.
牛结核病（bTB）主要由牛分枝杆菌（M. bovis）引起，是全球范围内家畜的一种主要经济疾病。疫苗接种被认为是当前控制策略的一种潜在可持续辅助手段。用减毒活牛分枝杆菌卡介苗（BCG）对牛进行疫苗接种可提供不同程度的保护；这种变异性的原因尚不清楚。吲哚胺2,3-双加氧酶（IDO）通过催化色氨酸，被认为在对结核分枝杆菌（M. tuberculosis）的免疫反应中具有免疫调节作用。在这项工作中，我们使用免疫组织化学和数字图像分析来评估在接种或未接种BCG然后用M. bovis攻击的牛的不同发育阶段肉芽肿中IDO的存在情况。我们的结果表明，未接种疫苗而受到M. bovis攻击的动物肉芽肿中IDO的表达高于接种BCG并受到M. bovis攻击的动物肉芽肿中的表达。因此，用BCG进行疫苗接种有可能阻止M. bovis诱导被认为是宿主免疫抑制途径的过程，而这些途径在疾病期间会导致病理变化。
