Tanini A, Brandi M L, Modigliani U, Rotella C M, Toccafondi R
Acta Endocrinol (Copenh). 1986 Nov;113(3):346-54. doi: 10.1530/acta.0.1130346.
TSH-induced cAMP accumulation in cells obtained from normal and pathological thyroid tissue was studied during the first 12 days of primary culture. In normal thyroid tissue cultures (N = 7), the response of cAMP to TSH was present from the second day of culture and reached its maximum after 8 days. A similar behaviour was observed in cultures obtained from euthyroid sporadic goitres (N = 8), even if the rate of response was slightly lower than that of normal tissue. Similarly, cultured cells from euthyroid 'autonomous' nodules (N = 8) appeared to be responsive to TSH during the period of study, but the rate of response was also lower than in the controls. On the contrary, in cultures obtained from toxic adenomas (N = 5) and from diffuse toxic goitres (N = 5) the response to TSH was absent during the first 4 days of culture. The cells became sensitive to TSH from 6 and 6 day onwards, with the rate of response increasing progressively and reaching its maximum on day 12. Finally, in cultured cells obtained from different areas of multinodular toxic goitres (N = 4), the response to TSH was similar to that of euthyroid goitres in cells prepared from 'cold' areas, and to that of toxic adenomas in cells obtained from 'hot' areas. The present data demonstrate the existence of an inhibitory action of unknown factors, possibly iodothyronines or thyroglobulin, on the TSH effect in short-term cultures obtained from thyrotoxic tissues. A normal TSH responsiveness can be restored when the culture is prolonged.
在原代培养的前12天，研究了促甲状腺激素（TSH）诱导的从正常和病理甲状腺组织获取的细胞中cAMP的积累情况。在正常甲状腺组织培养物中（N = 7），从培养的第二天开始就出现了cAMP对TSH的反应，并在8天后达到最大值。在来自甲状腺功能正常的散发性甲状腺肿的培养物中（N = 8）也观察到了类似的行为，即使反应速率略低于正常组织。同样，来自甲状腺功能正常的“自主性”结节的培养细胞（N = 8）在研究期间似乎对TSH有反应，但反应速率也低于对照组。相反，在来自毒性腺瘤（N = 5）和弥漫性毒性甲状腺肿的培养物中（N = 5），在培养的前4天对TSH没有反应。从第6天和第6天开始，细胞对TSH变得敏感，反应速率逐渐增加，并在第12天达到最大值。最后，在来自多结节毒性甲状腺肿不同区域的培养细胞中（N = 4），对TSH的反应类似于从“冷”区制备的甲状腺功能正常的甲状腺肿细胞中的反应，以及类似于从“热”区获得的细胞中毒性腺瘤的反应。目前的数据表明，在从甲状腺毒症组织获得的短期培养物中，存在未知因子（可能是碘甲状腺原氨酸或甲状腺球蛋白）对TSH作用的抑制作用。当培养时间延长时，可以恢复正常的TSH反应性。
