Campos M, Rossi C R
Am J Vet Res. 1986 Nov;47(11):2411-4.
Cytotoxic lymphocytes against infectious bovine rhinotracheitis virus (IBRV)-infected cells were induced by in vitro stimulation with IBRV antigen of peripheral blood leukocytes obtained from hyperimmune cattle. After one in vitro stimulation with IBRV, cytotoxicity was detected against IBRV-infected autologous target cells, but not against heterologous IBRV-infected or K562 target cells. After 4 in vitro IBRV stimulations at weekly intervals (in the presence of autologous feeder cells followed by interleukin 2 treatment at 2-day intervals), cytotoxicity was detected against autologous-, and to a lesser extent, heterologous-infected cells. Most cells in cytotoxic cultures were positive to a monoclonal antibody shown to react with bovine T lymphocytes.
用从超免疫牛获得的外周血白细胞的传染性牛鼻气管炎病毒（IBRV）抗原进行体外刺激，诱导出针对感染IBRV细胞的细胞毒性淋巴细胞。用IBRV进行一次体外刺激后，检测到对感染IBRV的自体靶细胞具有细胞毒性，但对异源感染IBRV的细胞或K562靶细胞没有细胞毒性。每周间隔进行4次体外IBRV刺激（在自体饲养细胞存在下，随后每隔2天进行白细胞介素2处理）后，检测到对自体感染细胞以及程度较轻的异源感染细胞具有细胞毒性。细胞毒性培养物中的大多数细胞对一种显示与牛T淋巴细胞反应的单克隆抗体呈阳性。
