Geder L, Lee K J, Dawson M S, Engle R, Maliniak R M, Lang C M
Am J Vet Res. 1981 Feb;42(2):300-7.
Infectious bovine rhinotracheitis virus (IBRV) established long-term persistent infection in intracerebrally inoculated athymic nude mice. After intraperitoneal injection into outbred mice, virus was isolated for only 3 days from spleens and livers. In vitro inoculation of outbred mouse spleen fragments with IBRV resulted in persistent infection and subsequent transformation of spleen macrophages. The IBRV-specific membrane and intracellular antigens were detected by indirect immunofluorescence techniques in transformed cells in early in vitro passages. The presence of IBRV genetic formation was confirmed by in situ hybridization. The IBRV-transformed mouse macrophages induced fibrosarcomas and cystic tumors in athymic nude mice. Infective virus could not be rescued from transformed cells by cocultivation with rabbit kidney cells, treatment with iododeoxyuridine, or ultraviolet irradiation.
传染性牛鼻气管炎病毒（IBRV）在经脑内接种的无胸腺裸鼠中建立了长期持续性感染。将其腹腔注射到远交系小鼠体内后，仅在脾脏和肝脏中分离到3天的病毒。用IBRV体外接种远交系小鼠脾脏片段导致持续性感染以及随后脾脏巨噬细胞的转化。在体外传代早期，通过间接免疫荧光技术在转化细胞中检测到了IBRV特异性膜抗原和细胞内抗原。通过原位杂交证实了IBRV基因结构的存在。IBRV转化的小鼠巨噬细胞在无胸腺裸鼠中诱导产生了纤维肉瘤和囊性肿瘤。通过与兔肾细胞共培养、用碘脱氧尿苷处理或紫外线照射，无法从转化细胞中拯救出感染性病毒。
