D'Errico A, Biagini G, Garbisa S, Milani M, Rizzoli R, Vasi V, Villanacci V, Grigioni W F, Mancini A M
Basic Appl Histochem. 1986;30(3):325-32.
An avidin-biotin-peroxidase labelling method was applied to frozen sections and to routine-fixed, paraffin-embedded sections, and compared with immunohistochemical results of stromal antigen detection (type IV collagen, laminin and fibronectin) obtained in parallel on different neoplasias. Lung, breast and gastrointestinal tract tumors were studied. Superimposable sections were obtained from cryostat and formalin-fixed, paraffin-embedded specimens (previously digested by pepsin). The data demonstrate the potential use of immunohistochemical investigation of paraffin-embedded tissues for histological analyses of tumors.
将抗生物素蛋白-生物素-过氧化物酶标记方法应用于冰冻切片以及常规固定的石蜡包埋切片，并与在不同肿瘤上同时获得的基质抗原检测（IV型胶原、层粘连蛋白和纤连蛋白）的免疫组化结果进行比较。研究了肺、乳腺和胃肠道肿瘤。从低温恒温器以及福尔马林固定的石蜡包埋标本（先前用胃蛋白酶消化）获得了叠加切片。数据证明了对石蜡包埋组织进行免疫组化研究在肿瘤组织学分析中的潜在用途。
