University of California, Berkeley, CA, USA.
J Biol Rhythms. 2018 Dec;33(6):567-588. doi: 10.1177/0748730418795802. Epub 2018 Sep 24.
Endocrine research in animals often entails exogenous hormone administration. Special issues arise when developing administration protocols for hormones with circadian and seasonal periodicity. This article reviews various methods for the exogenous administration of hormones with such periodicities by focusing on melatonin. We discuss that methodological variations across studies can affect experimental results. Melatonin administration techniques used in vertebrates includes infusion pumps, beeswax pellets, oral administration, injections, SILASTIC capsules, osmotic pumps, transdermal delivery, beads, and sponges.
动物内分泌研究通常需要外源激素给药。在制定具有昼夜节律和季节性周期性的激素给药方案时，会出现一些特殊问题。本文通过关注褪黑素，综述了具有此类周期性的外源激素给药的各种方法。我们讨论了研究中方法学的差异会如何影响实验结果。在脊椎动物中，褪黑素给药技术包括输注泵、蜂蜡丸、口服、注射、SILASTIC 胶囊、渗透泵、透皮给药、微球和海绵。
