Paikkatt Jophie Varghese, Aslam Sachin, Sreedharan Sheela, Philomina Beena, Kannan V P, Madhu S
Department of Pedodontics, MES Dental College, Malappuram, Kerala, India.
Department of Oral Surgery, MES Dental College, Malappuram, Kerala, India.
J Indian Soc Pedod Prev Dent. 2018 Jul-Sep;36(3):268-272. doi: 10.4103/JISPPD.JISPPD_152_17.
The aim of this in vivo study is to evaluate the antimicrobial efficacy of calcium hydroxide (Ca(OH)), chlorhexidine (CHX), and metronidazole gel as intracanal medicaments against aerobic and facultative anaerobic microorganisms found in root canals of human primary teeth with necrotic pulp.
It is a double-blinded randomized clinical trial.
Pulp canals of 45 single-rooted primary maxillary anterior teeth with pulp necrosis in 34 children were included in the study. They were divided into three groups of 15 samples each: Group I: Ca(OH); Group II: 1% CHX gel; and Group III: 1% metronidazole gel. Microbial count was obtained from each tooth at two different stages - (1) after instrumentation and (2) after placement of the medication. Statistical analysis using the SPSS 10.0 software program (SPSS Inc., Chicago, IL, USA) with Wilcoxon signed-rank test after grouping the samples was performed.
Ca(OH), 1% CHX gel, and 1% metronidazole gel were ineffective in completely eliminating aerobic and facultative anaerobic microorganism from root canal of human primary teeth with necrotic pulp.
None of the commonly used intracanal medicaments, that is Ca(OH), 1% CHX gel, and 1% metronidazole gel, was effective in completely eliminating aerobic and facultative anaerobic microorganism from root canal of human primary teeth with necrotic pulp. Ineffectiveness of these medicaments against aerobic and facultative anaerobic microorganism has opened new door of research regarding the manner of bacterial growth in unfavorable environmental and nutritional conditions, the way root canal environment favors biofilm formation and the use of suitable intracanal medicaments against single and multispecies biofilms.
本体内研究旨在评估氢氧化钙（Ca(OH)）、氯己定（CHX）和甲硝唑凝胶作为根管内药物对人类乳牙牙髓坏死根管中需氧菌和兼性厌氧菌的抗菌效果。
这是一项双盲随机临床试验。
本研究纳入了34名儿童的45颗单根上颌乳前牙，这些牙齿均有牙髓坏死。将其分为三组，每组15个样本：第一组：Ca(OH)；第二组：1% CHX凝胶；第三组：1%甲硝唑凝胶。在两个不同阶段从每颗牙齿获取微生物计数——（1）器械操作后，（2）药物放置后。样本分组后使用SPSS 10.0软件程序（美国伊利诺伊州芝加哥市SPSS公司）进行Wilcoxon符号秩检验的统计分析。
Ca(OH)、1% CHX凝胶和1%甲硝唑凝胶在完全清除人类乳牙牙髓坏死根管中的需氧菌和兼性厌氧菌方面均无效。
常用的根管内药物，即Ca(OH)、1% CHX凝胶和1%甲硝唑凝胶，均无法有效完全清除人类乳牙牙髓坏死根管中的需氧菌和兼性厌氧菌。这些药物对需氧菌和兼性厌氧菌的无效性为关于细菌在不利环境和营养条件下的生长方式、根管环境促进生物膜形成的方式以及使用合适的根管内药物对抗单一和多种生物膜的研究打开了新的大门。
