Chandna Preetika, Srivastava Nikhil, Sharma Alpana, Sharma Vrinda, Gupta Nidhi, Adlakha Vivek Kumar
Department of Pedodontics and Preventive Dentistry, Subharti Dental College, Swami Vivekanand Subharti University, Meerut, Uttar Pradesh, India.
Department of Biochemistry, All India Institute of Medical Sciences, New Delhi, India.
J Indian Soc Pedod Prev Dent. 2018 Jul-Sep;36(3):290-295. doi: 10.4103/JISPPD.JISPPD_225_17.
This study aimed to quantitatively assess the levels of Scardovia wiggsiae in caries-free and early childhood caries (ECC)- and severe ECC (SECC)-affected children using real-time polymerase chain reaction (RT-PCR).
Forty-five children aged <71 months were randomly recruited from the Outpatient Clinic at the Department of Pedodontics and Preventive Dentistry at Subharti Dental College and Hospital, Meerut, India. Fifteen children suffering from ECC, 15 with SECC, and 15 children without ECC were enrolled in the study. About 1-2 mL of unstimulated saliva was collected and subjected to microbial analysis using RT-PCR.
The SECC group (n = 15) was found to have significantly higher mean relative 16s rRNA expression of S. wiggsiae (3.67) than both ECC (n = 15) and controls (n = 15) (1.69 and 0.85, respectively). S. wiggsiae was detected in 86.7% of the SECC and 60% ECC group and was detected negligibly in the control (caries free) group. The correlation of decayed, missing, or filled surface levels with 16s rRNA levels showed significant positive correlation with 16S rRNA in both ECC and SECC patients.
Salivary levels of S. wiggsiae were significantly associated with ECC in children. S. wiggsiae represents a new frontier in the microbial etiology of ECC. This may lead to the development of new antimicrobial agents targeted to this organism and improve the treatment of ECC.
本研究旨在使用实时聚合酶链反应（RT-PCR）定量评估无龋儿童以及患幼儿龋（ECC）和重度幼儿龋（SECC）儿童中威格斯嘉德氏菌（Scardovia wiggsiae）的水平。
从印度密鲁特苏巴蒂牙科学院和医院儿童牙科学与预防牙科学门诊随机招募45名年龄小于71个月的儿童。15名患ECC的儿童、15名患SECC的儿童以及15名无ECC的儿童被纳入研究。收集约1-2 mL未刺激唾液，并使用RT-PCR进行微生物分析。
发现SECC组（n = 15）中威格斯嘉德氏菌的平均相对16s rRNA表达水平（3.67）显著高于ECC组（n = 15）和对照组（n = 15）（分别为1.69和0.85）。在86.7%的SECC组和60%的ECC组中检测到威格斯嘉德氏菌，而在对照组（无龋）中检测到的数量可忽略不计。龋失补牙面水平与16s rRNA水平的相关性显示，ECC和SECC患者中16S rRNA均呈显著正相关。
儿童唾液中威格斯嘉德氏菌水平与ECC显著相关。威格斯嘉德氏菌是ECC微生物病因学中的一个新领域。这可能会促使开发针对该微生物的新型抗菌剂，并改善ECC的治疗。
