São Paulo State University (UNESP), School of Dentistry, Araçatuba, Department of Pediatric Dentistry and Public Health, Araçatuba, SP, Brazil.
The Forsyth Institute, Department of Microbiology, Cambridge, MA, USA.
Arch Oral Biol. 2017 Nov;83:282-288. doi: 10.1016/j.archoralbio.2017.08.006. Epub 2017 Aug 16.
This study aimed to assess differences in selected oral bacteria in children according to the severity of dental caries. One hundred and thirty-six children, 36-60 months old were divided into three groups according to caries status: caries-free (CF) (n=47), early childhood caries (ECC) (n=40) and severe-early childhood caries (S-ECC) (n=49). Saliva was collected for detection and quantification of selected oral streptococci, Actinomyces naeslundii, Lactobacillus spp., Bifidobacterium spp., and Scardovia wiggsiae by quantitative-polymerase chain reaction. The results showed that the detection and quantitative levels of S. mutans, S. sobrinus, Bifidobacterium spp. and S. wiggsiae were significantly higher in S-ECC children compared to CF and ECC children, while for S. salivarius were significantly higher in CF compared to ECC and S-ECC children. There was no statistical difference among the clinical groups for S. mitis, S. oralis, A. naeslundii and Lactobacillus spp. levels and detection. S-ECC children had a lower monthly family income, started tooth brushing later and were breastfeed for a longer duration compared to CF children. S. mutans levels were positively correlated with S. wiggsiae and Bifidobacterium spp. levels, lower mother's education and child bottle-feeding before sleeping and negatively correlated with S. salivarius. It was concluded that in addition to S. mutans, other bacterial species, including bifidobacteria, Scardovia wiggsiae and S. sobrinus, are associated with severity of early childhood caries, although their role in the progress of dental caries remains unclear.
本研究旨在评估根据龋齿严重程度，儿童口腔中选定细菌的差异。将 136 名 36-60 个月大的儿童根据龋齿状况分为三组：无龋齿（CF）（n=47）、幼儿龋齿（ECC）（n=40）和严重幼儿龋齿（S-ECC）（n=49）。收集唾液，通过定量聚合酶链反应检测和定量选定的口腔链球菌、奈瑟氏放线菌、乳杆菌属、双歧杆菌属和 Scardovia wiggsiae。结果表明，与 CF 和 ECC 儿童相比，S-ECC 儿童的 S. mutans、S. sobrinus、Bifidobacterium spp. 和 S. wiggsiae 的检测和定量水平明显更高，而 S. salivarius 在 CF 儿童中明显高于 ECC 和 S-ECC 儿童。S. mitis、S. oralis、A. naeslundii 和乳杆菌属的水平和检测在临床组之间无统计学差异。与 CF 儿童相比，S-ECC 儿童的家庭月收入较低、刷牙开始较晚、母乳喂养时间较长。S. mutans 水平与 S. wiggsiae 和 Bifidobacterium spp. 水平呈正相关，与母亲的教育水平较低、睡前奶瓶喂养呈负相关，与 S. salivarius 呈负相关。研究结论：除了 S. mutans 之外，其他细菌种类，包括双歧杆菌、Scardovia wiggsiae 和 S. sobrinus，与幼儿龋齿的严重程度有关，尽管它们在龋齿进展中的作用尚不清楚。
