大肠杆菌核糖体蛋白S1不能保护16S rRNA的49个核苷酸的3'末端嗜杀素片段免受核酸酶S1的作用。
Escherichia coli ribosomal protein S1 does not protect the 49-nucleotide 3' terminal cloacin fragment of 16 S rRNA from nuclease S1.
作者信息
Wickstrom E
出版信息
Biochim Biophys Acta. 1986 Dec 18;868(4):265-9. doi: 10.1016/0167-4781(86)90064-3.
PMID:3024724
Abstract
Footprinting of ribosomal protein S1 on the 49-nucleotide 3' terminal cloacin DF13 fragment of 16 S rRNA at physiological ionic strength, pH and temperature yielded no detectable protection of any nucleotides from subsequent attack by the single strand specific nuclease S1, even at large excesses of ribosomal protein S1.
摘要
在生理离子强度、pH值和温度条件下，对核糖体蛋白S1与16S rRNA的49个核苷酸3'末端杀稻瘟菌素DF13片段进行足迹分析，即使核糖体蛋白S1大量过量，也未检测到任何核苷酸受到保护而免受单链特异性核酸酶S1的后续攻击。
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