a Department of Environmental Health School of Public Health and the Key Laboratory of Public Health Safety Ministry of Education , Fudan University , Shanghai , China.
b Shanghai Changning Center for Disease Control and Prevention, Shanghai, China.
Inhal Toxicol. 2018 May;30(6):239-246. doi: 10.1080/08958378.2018.1508258. Epub 2018 Sep 24.
Lots of epidemiological and experimental studies have found that ambient fine particulate matter (PM) exposure is associated with the development of cardiopulmonary diseases, obesity and diabetes. This study focused on the effects of cumulative PM exposure on pulmonary and systemic inflammation and insulin resistance. Thirty-two 6-week-old male Balb/c mice were randomly divided into four groups (FA, PM, WEEK and DAY groups) and were continuously or intermittently exposed to concentrated PM or filtered air (FA) for four weeks using Shanghai Meteorological and Environmental Animal Exposure System ("Shanghai-METAS"). The levels of IL-6 and TNF-α in serum, bronchoalveolar lavage fluid (BALF), lung tissues and white adipose tissue (WAT) were measured. Meanwhile, the expression of NF-κB and phosphor-NF-κB in lung tissue was detected by Western blot. Glucose tolerance and insulin resistance were also determined at the end of exposure. The results found that the mice in PM group displayed moderate inflammatory cell infiltration in lung, whereas the mice in WEEK and DAY groups displayed slight inflammatory cell infiltration in lung. Compared with the mice in FA group, the mRNA expressions of IL-6 and TNF-α in lung tissue and WAT significantly increased in the mice of PM group. Importantly, IL-6 and TNF-α mRNA expressions in PM group were higher than those in WEEK and DAY groups. The protein expression of phospho-NF-κB in lung tissue showed that PM group showed the activation of NF-κB, which was higher than that in the WEEK and DAY groups. Meanwhile, the mice in PM group showed more severe glucose tolerance and insulin resistance than that in the WEEK and DAY groups. The results suggested that the reduction of PM cumulative exposure may alleviate pulmonary and adipose inflammation, insulin resistance and glucose tolerance impairment. The results provided a clue that the interruption of ambient PM exposures by systems such as indoor air purification could be of benefit to people's health.
大量的流行病学和实验研究表明，环境细颗粒物（PM）暴露与心肺疾病、肥胖和糖尿病的发展有关。本研究重点关注累积 PM 暴露对肺部和全身炎症以及胰岛素抵抗的影响。32 只 6 周龄雄性 Balb/c 小鼠被随机分为四组（FA、PM、WEEK 和 DAY 组），并使用上海气象环境动物暴露系统（“上海-METAS”）连续或间歇地暴露于浓缩 PM 或过滤空气（FA）四周。测量血清、支气管肺泡灌洗液（BALF）、肺组织和白色脂肪组织（WAT）中 IL-6 和 TNF-α 的水平。同时，通过 Western blot 检测肺组织中 NF-κB 和磷酸化 NF-κB 的表达。暴露结束时还测定了葡萄糖耐量和胰岛素抵抗。结果发现，PM 组小鼠肺部出现中度炎症细胞浸润，而 WEEK 和 DAY 组小鼠肺部仅出现轻度炎症细胞浸润。与 FA 组相比，PM 组小鼠肺组织和 WAT 中 IL-6 和 TNF-α 的 mRNA 表达明显增加。重要的是，PM 组的 IL-6 和 TNF-α mRNA 表达高于 WEEK 和 DAY 组。肺组织磷酸化 NF-κB 蛋白表达显示 PM 组 NF-κB 激活，高于 WEEK 和 DAY 组。同时，PM 组小鼠的葡萄糖耐量和胰岛素抵抗比 WEEK 和 DAY 组更严重。结果表明，减少 PM 累积暴露可能会减轻肺部和脂肪组织炎症、胰岛素抵抗和葡萄糖耐量损害。结果提示，室内空气净化等系统中断环境 PM 暴露可能有益于人们的健康。
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