深入难以触及之处，探寻生长缓慢细菌的脉搏。
Getting Our Fingers on the Pulse of Slow-Growing Bacteria in Hard-To-Reach Places.
机构信息
Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA.
Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA
出版信息
J Bacteriol. 2018 Nov 26;200(24). doi: 10.1128/JB.00540-18. Print 2018 Dec 15.
Chronic infections with slow-growing pathogens have plagued humans throughout history. However, assessing the identities and growth rates of bacteria in an infection has remained an elusive goal. Neubauer et al. (J. Bacteriol. 200:e00365-18, 2018, https://doi.org/10.1128/JB.00365-18) combine two cutting-edge approaches to make progress on both fronts: probing specific RNA molecules to assess the identity of actively transcribing microbes and measuring growth rates through incorporation of stable isotope labels. They found that growth rates of pathogens were relatively stable during antibacterial therapy. The article delves into a basic and unanswered question that gets to the heart of understanding infection: what are the microbial growth rates?
慢性感染缓慢生长的病原体一直困扰着人类的历史。然而，评估感染中细菌的身份和生长速度仍然是一个难以捉摸的目标。Neubauer 等人（J. Bacteriol. 200:e00365-18, 2018, https://doi.org/10.1128/JB.00365-18）结合了两种最先进的方法，在这两个方面都取得了进展：探测特定的 RNA 分子以评估活跃转录微生物的身份，并通过掺入稳定同位素标记来测量生长速度。他们发现，在抗菌治疗期间，病原体的生长速度相对稳定。这篇文章深入探讨了一个基本的、未得到解答的问题，这个问题是理解感染的核心：微生物的生长速度是多少？
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