Ohmine Takahito, Narai Seika, Matsubara Toshiki, Nomura Toshihito, Oda Kosuke, Fukushi Masaya, Irie Takashi, Komatsu Takayuki, Tohya Yukinobu, Sakaguchi Takemasa
Department of Virology, Graduate School of Biomedical and Health Sciences, Hiroshima University.
Department of Microbiology and Immunology, Aichi Medical University School of Medicine.
Biocontrol Sci. 2018;23(3):145-149. doi: 10.4265/bio.23.145.
Feline calicivirus (FCV) is frequently used as a surrogate of human norovirus. We investigated eligibility of FCV for anti-viral assay by investigating the stability of infectivity and pH sensitivity in comparison with other viruses. We found that infectivities of FCV and murine norovirus (MNV) are relatively unstable in infected cells compared with those of coxsackievirus (CoV) and poliovirus (PoV) , suggesting that FCV and MNV have vulnerability. Western blotting indicated that inactivation of FCV was not due to viral protein degradation. We also demonstrated sensitivity of FCV to low pH, the 50% inhibitory pH value being ca. 3.9. Since human norovirus is thought to persist longer, in infectivity and to be a resistant virus, CoV, which is robust and not restrained in use as PoV, may be more appropriate as a test virus for disinfectants, rather than FCV and MNV.
猫杯状病毒（FCV）常被用作人类诺如病毒的替代物。我们通过研究其与其他病毒相比的感染性稳定性和pH敏感性，来调查FCV用于抗病毒检测的适用性。我们发现，与柯萨奇病毒（CoV）和脊髓灰质炎病毒（PoV）相比，FCV和鼠诺如病毒（MNV）在感染细胞中的感染性相对不稳定，这表明FCV和MNV具有脆弱性。蛋白质免疫印迹法表明，FCV的失活并非由于病毒蛋白降解。我们还证明了FCV对低pH敏感，50%抑制pH值约为3.9。由于人们认为人类诺如病毒在感染性方面持续时间更长且是一种抗性病毒，CoV稳健且在使用上不像PoV那样受限，因此作为消毒剂的测试病毒，CoV可能比FCV和MNV更合适。
