• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

格里菲辛角叉菜胶速溶贴片可预防体内 SHIV、HSV-2 和 HPV 感染。

Griffithsin carrageenan fast dissolving inserts prevent SHIV HSV-2 and HPV infections in vivo.

机构信息

Center for Biomedical Research, Population Council, 1230 York Ave, New York, NY, 10065, USA.

PATH, 2201 Westlake Ave, Suite 200, Seattle, WA, 98121, USA.

出版信息

Nat Commun. 2018 Sep 24;9(1):3881. doi: 10.1038/s41467-018-06349-0.

DOI:10.1038/s41467-018-06349-0
PMID:30250170
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6155161/
Abstract

Human immunodeficiency virus (HIV) pre-exposure prophylaxis (PrEP) strategies with proven in vivo efficacy rely on antiretroviral drugs, creating the potential for drug resistance and complicated treatment options in individuals who become infected. Moreover, on-demand products are currently missing from the PrEP development portfolio. Griffithsin (GRFT) is a non-antiretroviral HIV entry inhibitor derived from red algae with an excellent safety profile and potent activity in vitro. When combined with carrageenan (CG), GRFT has strong activity against herpes simplex virus-2 (HSV-2) and human papillomavirus (HPV) in vitro and in vivo. Here, we report that GRFT/CG in a freeze-dried fast dissolving insert (FDI) formulation for on-demand use protects rhesus macaques from a high dose vaginal SHIV SF162P3 challenge 4 h after FDI insertion. Furthermore, the GRFT/CG FDI also protects mice vaginally against HSV-2 and HPV pseudovirus. As a safe, potent, broad-spectrum, on-demand non-antiretroviral product, the GRFT/CG FDI warrants clinical development.

摘要

人类免疫缺陷病毒（HIV）暴露前预防（PrEP）策略依赖于已被证实具有体内疗效的抗逆转录病毒药物，这为感染的个体带来了耐药性和复杂治疗选择的潜在风险。此外，按需产品目前在 PrEP 开发组合中缺失。Griffithsin（GRFT）是一种源自红藻的非抗逆转录病毒 HIV 进入抑制剂，具有出色的安全性和体外强效活性。当与角叉菜胶（CG）联合使用时，GRFT 在体外和体内对单纯疱疹病毒-2（HSV-2）和人乳头瘤病毒（HPV）具有强大的活性。在这里，我们报告说，GRFT/CG 在一种用于按需使用的冻干速溶插入物（FDI）配方中，可在 FDI 插入后 4 小时保护恒河猴免受高剂量阴道 SHIV SF162P3 挑战。此外，GRFT/CG FDI 还可保护小鼠阴道免受 HSV-2 和 HPV 假病毒的侵害。作为一种安全、强效、广谱、按需的非抗逆转录病毒产品，GRFT/CG FDI 值得临床开发。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/39c00762b560/41467_2018_6349_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/1956e83f1c25/41467_2018_6349_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/e67de52ffbd5/41467_2018_6349_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/6c5045c7cac8/41467_2018_6349_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/cedcba26fbea/41467_2018_6349_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/39c00762b560/41467_2018_6349_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/1956e83f1c25/41467_2018_6349_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/e67de52ffbd5/41467_2018_6349_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/6c5045c7cac8/41467_2018_6349_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/cedcba26fbea/41467_2018_6349_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8260/6155161/39c00762b560/41467_2018_6349_Fig5_HTML.jpg

相似文献

1
Griffithsin carrageenan fast dissolving inserts prevent SHIV HSV-2 and HPV infections in vivo.格里菲辛角叉菜胶速溶贴片可预防体内 SHIV、HSV-2 和 HPV 感染。
Nat Commun. 2018 Sep 24;9(1):3881. doi: 10.1038/s41467-018-06349-0.
2
Griffithsin and Carrageenan Combination To Target Herpes Simplex Virus 2 and Human Papillomavirus.格里菲斯菌素与卡拉胶联合用于靶向单纯疱疹病毒2型和人乳头瘤病毒
Antimicrob Agents Chemother. 2015 Dec;59(12):7290-8. doi: 10.1128/AAC.01816-15. Epub 2015 Sep 14.
3
A potent combination microbicide that targets SHIV-RT, HSV-2 and HPV.一种强效的组合型杀微生物剂，针对 SHIV-RT、HSV-2 和 HPV。
PLoS One. 2014 Apr 16;9(4):e94547. doi: 10.1371/journal.pone.0094547. eCollection 2014.
4
A modified zinc acetate gel, a potential nonantiretroviral microbicide, is safe and effective against simian-human immunodeficiency virus and herpes simplex virus 2 infection in vivo.一种改良的醋酸锌凝胶，一种有潜力的非抗逆转录病毒杀微生物剂，在体内对猴免疫缺陷病毒和单纯疱疹病毒 2 感染具有安全性和有效性。
Antimicrob Agents Chemother. 2013 Aug;57(8):4001-9. doi: 10.1128/AAC.00796-13. Epub 2013 Jun 10.
5
Short communication: a repeated simian human immunodeficiency virus reverse transcriptase/herpes simplex virus type 2 cochallenge macaque model for the evaluation of microbicides.简短通讯：一种用于评估杀微生物剂的重复感染猿猴人类免疫缺陷病毒逆转录酶/单纯疱疹病毒2型的猕猴模型
AIDS Res Hum Retroviruses. 2014 Nov;30(11):1117-24. doi: 10.1089/aid.2014.0207.
6
A combination microbicide gel protects macaques against vaginal simian human immunodeficiency virus-reverse transcriptase infection, but only partially reduces herpes simplex virus-2 infection after a single high-dose cochallenge.一种组合型杀微生物剂凝胶可保护猕猴免受阴道猿猴人类免疫缺陷病毒逆转录酶感染，但在单次高剂量共同攻击后，仅能部分降低单纯疱疹病毒2型感染。
AIDS Res Hum Retroviruses. 2014 Feb;30(2):174-83. doi: 10.1089/aid.2013.0165. Epub 2013 Nov 22.
7
An intravaginal ring that releases three antiviral agents and a contraceptive blocks SHIV-RT infection, reduces HSV-2 shedding, and suppresses hormonal cycling in rhesus macaques.一种阴道环，能释放三种抗病毒药物和一种避孕药，可阻断 SHIV-RT 感染，减少 HSV-2 脱落，并抑制恒河猴的激素循环。
Drug Deliv Transl Res. 2017 Dec;7(6):840-858. doi: 10.1007/s13346-017-0389-0.
8
Zinc acetate/carrageenan gels exhibit potent activity in vivo against high-dose herpes simplex virus 2 vaginal and rectal challenge.醋酸锌/卡拉胶凝胶对大剂量单纯疱疹病毒 2 阴道和直肠攻击具有显著的体内活性。
Antimicrob Agents Chemother. 2012 Jan;56(1):358-68. doi: 10.1128/AAC.05461-11. Epub 2011 Nov 7.
9
Development of a Vaginal Fast-Dissolving Insert Combining Griffithsin and Carrageenan for Potential Use Against Sexually Transmitted Infections.开发一种结合 Griffithsin 和卡拉胶的阴道速溶插入物，用于潜在对抗性传播感染。
J Pharm Sci. 2018 Oct;107(10):2601-2610. doi: 10.1016/j.xphs.2018.06.002. Epub 2018 Jun 11.
10
Results of a phase 1, randomized, placebo-controlled first-in-human trial of griffithsin formulated in a carrageenan vaginal gel.卡波姆阴道凝胶配方 Griffithsin 的 1 期、随机、安慰剂对照的首次人体试验结果。
PLoS One. 2022 Jan 20;17(1):e0261775. doi: 10.1371/journal.pone.0261775. eCollection 2022.

引用本文的文献

1
Isolation and structure elucidation of Dm-CVNH, a new cyanovirin-N homolog with activity against SARS-CoV-2 and HIV-1.Dm-CVNH的分离与结构解析，一种对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）和人类免疫缺陷病毒1型（HIV-1）具有活性的新型氰苷蛋白N（cyanovirin-N）同源物。
J Biol Chem. 2025 Mar;301(3):108319. doi: 10.1016/j.jbc.2025.108319. Epub 2025 Feb 14.
2
Expression and antiviral application of exogenous lectin (griffithsin) in sweetpotatoes.外源凝集素（格里菲斯菌素）在甘薯中的表达及抗病毒应用
Front Plant Sci. 2024 Jul 16;15:1421244. doi: 10.3389/fpls.2024.1421244. eCollection 2024.
3
Preclinical and Early Clinical Development of Tenofovir Alafenamide/Elvitegravir Topical Inserts for Effective On-Demand Vaginal and Rectal HIV Prevention.

本文引用的文献

1
Development of a Vaginal Fast-Dissolving Insert Combining Griffithsin and Carrageenan for Potential Use Against Sexually Transmitted Infections.开发一种结合 Griffithsin 和卡拉胶的阴道速溶插入物，用于潜在对抗性传播感染。
J Pharm Sci. 2018 Oct;107(10):2601-2610. doi: 10.1016/j.xphs.2018.06.002. Epub 2018 Jun 11.
2
Efficacy of a Carrageenan gel Against Transmission of Cervical HPV (CATCH): interim analysis of a randomized, double-blind, placebo-controlled, phase 2B trial.卡波姆凝胶预防宫颈 HPV 感染（CATCH）的疗效：一项随机、双盲、安慰剂对照、2B 期临床试验的中期分析。
Clin Microbiol Infect. 2019 Feb;25(2):210-216. doi: 10.1016/j.cmi.2018.04.012. Epub 2018 Apr 21.
3
用于按需有效预防阴道和直肠HIV感染的替诺福韦艾拉酚胺/elvitegravir局部插入剂的临床前和早期临床开发。
Pharmaceutics. 2024 Mar 1;16(3):348. doi: 10.3390/pharmaceutics16030348.
4
Sulfated Polysaccharides from Seaweeds: A Promising Strategy for Combatting Viral Diseases-A Review.海藻硫酸多糖：对抗病毒性疾病的一种有前景的策略——综述
Mar Drugs. 2023 Aug 23;21(9):461. doi: 10.3390/md21090461.
5
Delivery of biologics: Topical administration.生物制剂的传递：局部给药。
Biomaterials. 2023 Nov;302:122312. doi: 10.1016/j.biomaterials.2023.122312. Epub 2023 Sep 4.
6
Sustained Delivery of the Antiviral Protein Griffithsin and Its Adhesion to a Biological Surface by a Silk Fibroin Scaffold.通过丝素蛋白支架实现抗病毒蛋白格里菲斯菌素的持续递送及其在生物表面的粘附
Materials (Basel). 2023 Aug 9;16(16):5547. doi: 10.3390/ma16165547.
7
Application of natural polysaccharides and their novel dosage forms in gynecological cancers: therapeutic implications from the diversity potential of natural compounds.天然多糖及其新型剂型在妇科癌症中的应用：天然化合物多样潜力的治疗意义
Front Pharmacol. 2023 Jun 13;14:1195104. doi: 10.3389/fphar.2023.1195104. eCollection 2023.
8
A Brief History and Advancement of Contraceptive Multipurpose Prevention Technology (cMPT) Products.避孕多功能预防技术（cMPT）产品的简史与进展
Open Access J Contracept. 2023 Jun 13;14:83-94. doi: 10.2147/OAJC.S375634. eCollection 2023.
9
A topical rectal douche product containing Q-Griffithsin does not disrupt the epithelial border or alter CD4 cell distribution in the human rectal mucosa.一种含有 Q-格里菲辛的直肠局部冲洗剂不会破坏人类直肠黏膜的上皮边界或改变 CD4 细胞分布。
Sci Rep. 2023 May 9;13(1):7547. doi: 10.1038/s41598-023-34107-w.
10
A Systematic Review of Second-Line Treatments in Antiviral Resistant Strains of HSV-1, HSV-2, and VZV.对单纯疱疹病毒1型、单纯疱疹病毒2型和水痘-带状疱疹病毒抗病毒耐药株二线治疗的系统评价
Cureus. 2023 Mar 9;15(3):e35958. doi: 10.7759/cureus.35958. eCollection 2023 Mar.
Effect of HSV-2 infection on subsequent HIV acquisition: an updated systematic review and meta-analysis.
单纯疱疹病毒2型感染对后续获得性艾滋病病毒的影响：一项更新的系统评价与荟萃分析
Lancet Infect Dis. 2017 Dec;17(12):1303-1316. doi: 10.1016/S1473-3099(17)30405-X. Epub 2017 Aug 23.
4
Repeated administration of high-dose depot medroxyprogesterone acetate does not alter SHIV viral kinetics and tenofovir pharmacokinetics when delivered via intravaginal rings.当通过阴道环给药时，重复给予高剂量长效醋酸甲羟孕酮不会改变猴免疫缺陷病毒（SHIV）的病毒动力学和替诺福韦的药代动力学。
J Med Primatol. 2017 Aug;46(4):129-136. doi: 10.1111/jmp.12299.
5
An intravaginal ring that releases three antiviral agents and a contraceptive blocks SHIV-RT infection, reduces HSV-2 shedding, and suppresses hormonal cycling in rhesus macaques.一种阴道环，能释放三种抗病毒药物和一种避孕药，可阻断 SHIV-RT 感染，减少 HSV-2 脱落，并抑制恒河猴的激素循环。
Drug Deliv Transl Res. 2017 Dec;7(6):840-858. doi: 10.1007/s13346-017-0389-0.
6
On-demand microbicide products: design matters.按需杀微生物剂产品：设计很重要。
Drug Deliv Transl Res. 2017 Dec;7(6):775-795. doi: 10.1007/s13346-017-0385-4.
7
A Comparison of the Natural History of HPV Infection and Cervical Abnormalities among HIV-Positive and HIV-Negative Women in Senegal, Africa.非洲塞内加尔HIV阳性和HIV阴性女性人乳头瘤病毒（HPV）感染及宫颈异常自然史的比较
Cancer Epidemiol Biomarkers Prev. 2017 Jun;26(6):886-894. doi: 10.1158/1055-9965.EPI-16-0700. Epub 2017 May 17.
8
Pharmacokinetics of the Antiviral Lectin Griffithsin Administered by Different Routes Indicates Multiple Potential Uses.不同给药途径的抗病毒凝集素格里菲斯菌素的药代动力学表明其具有多种潜在用途。
Viruses. 2016 Dec 17;8(12):331. doi: 10.3390/v8120331.
9
In vitro inhibition of human papillomavirus following use of a carrageenan-containing vaginal gel.使用含角叉菜胶的阴道凝胶后对人乳头瘤病毒的体外抑制作用
Gynecol Oncol. 2016 Nov;143(2):313-318. doi: 10.1016/j.ygyno.2016.09.003. Epub 2016 Sep 10.
10
PolyICLC Exerts Pro- and Anti-HIV Effects on the DC-T Cell Milieu In Vitro and In Vivo.聚肌胞苷酸-聚左旋赖氨酸-羧甲基纤维素在体外和体内对树突状细胞- T细胞环境发挥促进和抗HIV作用。
PLoS One. 2016 Sep 7;11(9):e0161730. doi: 10.1371/journal.pone.0161730. eCollection 2016.