Chen Honglei, Yuan Kaitao, Wang Xinyou, Wang Huashe, Wu Qiuning, Wu Xiaobin, Peng Junsheng
Department of Gastrointestinal Endoscopy, The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, P.R. China.
Department of Gastrointestinal Surgery, The First Affiliated Hospital, Sun Yat-sen University, Yuexiu, Guangzhou, Guangdong 510080, P.R. China.
Oncol Lett. 2018 Oct;16(4):5419-5425. doi: 10.3892/ol.2018.9318. Epub 2018 Aug 17.
The present study aimed to investigate the expression, clinical association, and prognosis of RecQ protein-like 4 (RECQL4) protein in human gastric cancers (GCs). The expression levels and prognostic value of RECQL4 were initially predicted by using bioinformatics. GC specimens and matched normal gastric tissues were evaluated by immunohistochemistry (IHC), and patient clinicopathological parameters and survival times were analyzed. Multivariate Cox analysis was used to determine the prognostic role of RECQL4 expression. The Oncomine database predicted that RECQL4 mRNA expression levels were significantly increased in GCs as compared with those in normal gastric tissues (P<0.05) and that patients with increased RECQL4 mRNA expression levels had significantly lower overall survival (OS) (P<0.001). The results of IHC showed that the positive rate of RECQL4 in the GC samples was significantly higher than that in the normal gastric mucosa specimens (P<0.05). RECQL4 expression was positively associated with depth of invasion and TNM (P<0.05). High RECQL4 expression in GC samples was significantly associated with poor OS (P=0.024). Positive RECQL4 expression, depth of invasion, lymphatic invasion, and TNM staging were independent factors for predicting worse OS rates by using multivariate analysis. Compared with expression levels in normal gastric tissues, RECQL4 was significantly overexpressed in GC samples, and increased RECQL4 expression was an independent predictor of poor prognosis in GC patients.
本研究旨在探讨RecQ蛋白样4（RECQL4）蛋白在人类胃癌（GC）中的表达、临床相关性及预后情况。首先利用生物信息学预测RECQL4的表达水平及预后价值。通过免疫组织化学（IHC）评估GC标本及配对的正常胃组织，并分析患者的临床病理参数和生存时间。采用多因素Cox分析确定RECQL4表达的预后作用。Oncomine数据库预测，与正常胃组织相比，GC中RECQL4 mRNA表达水平显著升高（P<0.05），且RECQL4 mRNA表达水平升高的患者总生存期（OS）显著降低（P<0.001）。IHC结果显示，GC样本中RECQL4的阳性率显著高于正常胃黏膜标本（P<0.05）。RECQL4表达与浸润深度和TNM分期呈正相关（P<0.05）。GC样本中RECQL4高表达与较差的OS显著相关（P=0.024）。采用多因素分析，RECQL4阳性表达、浸润深度、淋巴浸润和TNM分期是预测OS率较差的独立因素。与正常胃组织中的表达水平相比，RECQL4在GC样本中显著过表达，且RECQL4表达增加是GC患者预后不良的独立预测因素。
