Onuma M, Odawara T, Watarai S, Aida Y, Ochiai K, Syuto B, Matsumoto K, Yasuda T, Fujimoto Y, Izawa H
Jpn J Cancer Res. 1986 Nov;77(11):1161-7.
Monoclonal antibody against tumor-associated antigen (TAA) expressed on bovine leukemia cells was conjugated to liposomes containing adriamycin (ADM), and the specificity and therapeutic effects of the conjugates were examined in vitro and in vivo using a TAA-positive bovine leukemia cell line as the target tumor. In vitro studies with the TAA-positive cell line clearly indicated that the antibody-conjugated liposomes containing ADM exerted selective effects on TAA-positive cells in the inhibition assay of 3H-thymidine incorporation. Three injections of liposomes containing ADM (4 mg/kg) into tumor-bearing nude mice significantly inhibited the tumor growth and the therapeutic effect of the antibody-conjugated liposomes was far greater than that of normal mouse IgG-conjugated liposomes as assessed in terms of tumor size.
将针对牛白血病细胞上表达的肿瘤相关抗原（TAA）的单克隆抗体与含有阿霉素（ADM）的脂质体偶联，并以TAA阳性牛白血病细胞系作为靶肿瘤，在体外和体内研究了偶联物的特异性和治疗效果。对TAA阳性细胞系的体外研究清楚地表明，在3H-胸腺嘧啶核苷掺入抑制试验中，含有ADM的抗体偶联脂质体对TAA阳性细胞具有选择性作用。向荷瘤裸鼠注射三次含有ADM（4mg/kg）的脂质体可显著抑制肿瘤生长，从肿瘤大小评估，抗体偶联脂质体的治疗效果远大于正常小鼠IgG偶联脂质体。
