血液透析患者乙型肝炎病毒疫苗接种后抗-HBs 效价与相对吲哚胺 2,3-双加氧酶转录水平的关系。
Relative indoleamine 2,3-dioxygenase transcript level concerning anti-HBs titers in response to HBV vaccination in hemodialysis patients.
机构信息
a Department of Nephrology , Transplantology and Internal Diseases, Poznan University of Medical Sciences , Poznań , Poland.
b Department of Biochemistry and Molecular Biology , Poznan University of Medical Sciences , Poznań , Poland.
出版信息
Expert Rev Vaccines. 2018 Oct;17(10):947-953. doi: 10.1080/14760584.2018.1527691. Epub 2018 Oct 1.
BACKGROUND
Indoleamine 2,3-dioxygenase (IDO) is a negative regulator of the immune system. To approach reasons of variability in the generation of anti-HBs antibodies in response to HBV vaccination among hemodialysis (HD) subjects, we aimed to investigate whether the IDO gene (IDO1) transcript levels are associated with post-vaccination anti-HBs production and IDO1 polymorphic variants.
METHODS
The IDO1 transcript was determined by qRT-PCR analysis in 110 HD patients. IDO1 (rs3739319, rs9657182) genotyping was carried out by HRM analysis.
RESULTS
The relative IDO1 transcript levels were not associated with IDO1 polymorphic variants. There were 16 non-responders (not able to produce anti-HBs >10 IU/L), 74 patients with anti-HBs 10-999 IU/L, and 20 hyperactive responders (anti-HBs ≥1000 IU/L). IDO1 transcript levels were different among these groups (0.832, 0.423-4.373; 1.114, 0.317-6.582; 0.680, 0.164-3.014; respectively, Kruskal-Wallis P = 0.024). Significance in IDO1 transcript was shown between anti-HBs titers 10-999 IU/L and ≥1000 IU/L (P = 0.020). IDO1 transcript level <0.743 indicated 3.38 (1.17-9.72) higher probability of hyperactive immunization (adjusted P = 0.005).
CONCLUSION
In HD patients, ability to generate anti-HBs is not associated with IDO1 transcript levels. Hyperactive anti-HBs responses occur in patients showing lower IDO1 transcript. The latter cannot be predictable by genotyping IDO1 rs3739319 or rs9657182.
背景
吲哚胺 2,3-双加氧酶 (IDO) 是免疫系统的负调节剂。为了探讨血液透析 (HD) 患者对乙型肝炎病毒 (HBV) 疫苗接种产生抗 -HBs 抗体的变异性原因，我们旨在研究 IDO 基因 (IDO1) 转录水平是否与接种后抗 -HBs 产生和 IDO1 多态性变异有关。
方法
通过 qRT-PCR 分析检测 110 例 HD 患者的 IDO1 转录。通过 HRM 分析进行 IDO1(rs3739319、rs9657182) 基因分型。
结果
相对 IDO1 转录水平与 IDO1 多态性变异无关。有 16 名无应答者 (不能产生 >10 IU/L 的抗 -HBs)，74 名患者抗 -HBs 为 10-999 IU/L，20 名高应答者 (抗 -HBs≥1000 IU/L)。这些组之间的 IDO1 转录水平不同 (0.832、0.423-4.373；1.114、0.317-6.582；0.680、0.164-3.014；分别，Kruskal-Wallis P=0.024)。抗 -HBs 滴度为 10-999 IU/L 和≥1000 IU/L 之间的 IDO1 转录有显著性差异 (P=0.020)。IDO1 转录水平 <0.743 表示高应答免疫的可能性高 3.38(1.17-9.72)(调整后的 P=0.005)。
结论
在 HD 患者中，产生抗 -HBs 的能力与 IDO1 转录水平无关。低 IDO1 转录的患者出现高活性抗 -HBs 反应。后者不能通过 IDO1 rs3739319 或 rs9657182 基因分型预测。
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