Institute of Nuclear Physics Polish Academy of Sciences, PL-31342 Krakow, Poland.
Institute of Nuclear Physics Polish Academy of Sciences, PL-31342 Krakow, Poland.
Biochim Biophys Acta Mol Basis Dis. 2018 Nov;1864(11):3659-3663. doi: 10.1016/j.bbadis.2018.07.006. Epub 2018 Jul 6.
Raman spectroscopy of erythrocytes provides detailed information about the structure and status of heme moiety, which can be used to provide new insights into molecular pathogenesis of several diseases. In this study, we present the first Raman spectroscopy investigations of the effect of hemoglobin oxygenation in the context of hypertensive disease. The experimental data was subjected to Logistic Regression, which indicated heme‑oxygenation status as an important risk factor alongside other clinical parameters. The 1605/1621 cm band ratio was selected as an optimal Raman metric for risk assessment and along with other band ratios (1583, 1639, 1310 cm) related to heme status and when combined with clinical data via logistic regression gave an Area Under the Curve (AUC) >0.95 for prehypertension risk prediction. The work demonstrates the feasibility of Raman spectroscopy to distinguish between prehypertensive and normotensive states. Simultaneously, it is implied that the etiology of the high blood pressure progression may be connected with the changes in hemoglobin oxygenation.
红细胞的拉曼光谱提供了关于血红素部分结构和状态的详细信息，可用于深入了解多种疾病的分子发病机制。在这项研究中，我们首次展示了血红蛋白氧合对高血压疾病影响的拉曼光谱研究。对实验数据进行了逻辑回归分析，表明血红素氧合状态与其他临床参数一样，是一个重要的风险因素。1605/1621 cm 波段比被选为风险评估的最佳拉曼指标，与其他与血红素状态相关的波段比（1583、1639、1310 cm）一起，通过逻辑回归与临床数据相结合，可对高血压前期风险进行预测，曲线下面积（AUC）大于 0.95。这项工作证明了拉曼光谱法区分高血压前期和正常血压状态的可行性。同时，这表明高血压进展的病因可能与血红蛋白氧合的变化有关。
