Kaewkrajay Chutima, Limtong Savitree
1Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
2Division of Microbiology, Faculty of Science and Technology, Phranakhon Si Ayutthaya Rajabhat University, Phranakhon Si Ayutthaya 13000, Thailand.
Int J Syst Evol Microbiol. 2018 Nov;68(11):3611-3614. doi: 10.1099/ijsem.0.003043. Epub 2018 Sep 25.
Strain DMKU13-2, representing a novel anamorphic yeast species in the class Microbotryomycetes, subphylum Puccinomycotina, phylum Basidiomycota, was isolated from a soft coral collected in the sea off Ko Mu island, Thailand. The phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region and the D1/D2 region of the large subunit ribosomal RNA (LSU rRNA) gene indicated that this strain was a novel species in the genus Spencerozyma and distant from Spencerozyma crocea, the only species of the genus. The novel species differed from the type of S. crocea (CBS 2029) by 5.2 % nucleotides (31 nucleotide substitutions out of 594 bp) in the D1/D2 region of the LSU rRNA gene and 9.0 % nucleotides (66 nucleotide substitutions out of 735 bp) in the ITS region. The name Spencerozyma siamensis sp. nov. is proposed. The type is DMKU13-2 (=CBS 14683=TBRC 7039). The MycoBank number is MB 824889.
菌株DMKU13 - 2是从泰国阁穆岛附近海域采集的软珊瑚中分离得到的，它代表担子菌门锈菌亚门微酵母纲中的一个新的无性型酵母物种。基于核糖体RNA大亚基（LSU rRNA）基因的内部转录间隔区（ITS）和D1/D2区的串联序列进行的系统发育分析表明，该菌株是Spencerozyma属中的一个新物种，与该属唯一的物种Spencerozyma crocea亲缘关系较远。该新物种与Spencerozyma crocea模式菌株（CBS 2029）相比，在LSU rRNA基因的D1/D2区有5.2%的核苷酸差异（594 bp中有31个核苷酸替换），在ITS区有9.0%的核苷酸差异（735 bp中有66个核苷酸替换）。提出新物种名为暹罗斯彭塞酵母（Spencerozyma siamensis sp. nov.）。模式菌株为DMKU13 - 2（=CBS 14683=TBRC 7039）。MycoBank编号为MB 824889。
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