Liu Xin-Zhan, Groenewald Marizeth, Boekhout Teun, Bai Feng-Yan
State Key Laboratory for Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, PR China.
Westerdijk Fungal Biodiversity Institute, Uppsalalaan 8, Utrecht, The Netherlands.
Int J Syst Evol Microbiol. 2017 Nov;67(11):4534-4540. doi: 10.1099/ijsem.0.002323. Epub 2017 Sep 21.
Nine anamorphic yeast strains isolated from various plant leaves collected in southern China were phylogenetically characterized based on sequences of the internal transcribed spacer (ITS) region, the D1/D2 domains of the large subunit (LSU) rRNA gene, the small subunit (SSU) rRNA gene, the two subunits of the RNA polymerase II gene (RPB1 and RPB2) and the translation elongation factor 1-α (TEF1). Phylogenetic analysis of the combined sequences of the six genes showed that the new strains formed a distinct clade in the class Microbotryomycetes but could not be assigned to any of the existing genera, families or orders of the class. Three separate groups were consistently resolved from the nine new strains based on the combined sequences of the six genes and single gene sequences of ITS, RPB1, RPB2 and TEF1. The results suggest that the nine yeast strains compared represent three novel species in a novel genus. The names Heitmania gen. nov. (MycoBank registration number MB819987), Heitmania litseae sp. nov. (MB820112, type strain CGMCC 2.5697=CBS 14756), Heitmania castanopsis sp. nov. (MB819988, CGMCC 2.5698=CBS 14750) and Heitmania elacocarpi sp. nov. (MB820113, CGMCC 2.5695=CBS 14752) are proposed for the new taxa.
从中国南方采集的各种植物叶片中分离出9株变形酵母菌株，基于内转录间隔区（ITS）序列、大亚基（LSU）rRNA基因的D1/D2结构域、小亚基（SSU）rRNA基因、RNA聚合酶II基因的两个亚基（RPB1和RPB2）以及翻译延伸因子1-α（TEF1）对其进行系统发育特征分析。对这6个基因的联合序列进行系统发育分析表明，这些新菌株在微球酵母菌纲中形成了一个独特的分支，但不能归入该纲现有的任何属、科或目。基于这6个基因的联合序列以及ITS、RPB1、RPB2和TEF1的单基因序列，从这9个新菌株中一致解析出3个独立的组。结果表明，所比较的这9株酵母菌株代表了一个新属中的3个新物种。提出了新分类单元的名称：新属Heitmania（MycoBank登记号MB819987），新种Heitmania litseae（MB820112，模式菌株CGMCC 2.5697 = CBS 14756）、新种Heitmania castanopsis（MB819988，CGMCC 2.5698 = CBS 14750）和新种Heitmania elacocarpi（MB820113，CGMCC 2.5695 = CBS 14752）。
