Present address: Department of Food Technology, Faculty of Engineering and Industrial Technology, Silpakorn University, Sanamchandra Palace Campus, Nakhon Pathom 73000, Thailand.
Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand.
Int J Syst Evol Microbiol. 2020 Jun;70(6):3673-3678. doi: 10.1099/ijsem.0.004219. Epub 2020 May 14.
The strain DMKU-XD44, representing an anamorphic novel yeast species, was isolated from soil collected in a peat swamp forest (PSF) area in Rayong Botanical Garden in eastern Thailand. On the basis of sequence analysis of the D1/D2 region of the large subunit (LSU) rRNA gene and the internal transcribed spacer (ITS) region, CBS 10309 was the most closely related species. The novel species differed from the type by 2.5 % (14 nucleotide substitutions) in the D1/D2 region of the LSU rRNA gene and by 8.0 % (40 nucleotide substitutions) in the ITS region. The results of a phylogenetic analysis, based on the combined sequences of the ITS region and the D1/D2 region, indicated that DMKU-XD44 represents a member of the clade in the (, , and ) and is phylogenetically distinct from other species of the genus in the clade. Therefore, DMKU-XD44 represents a novel species of the genus . The name f.a., sp. nov. is proposed. The holotype is DMKU-XD44, while the MycoBank number is MB 832816.
菌株 DMKU-XD44 代表一种无性型的新型酵母种，是从泰国东部罗勇植物园的泥炭沼泽森林（PSF）地区采集的土壤中分离出来的。基于大亚基（LSU）rRNA 基因的 D1/D2 区和内部转录间隔区（ITS）序列分析，CBS 10309 是最接近的相关种。该新型种与模式种在 LSU rRNA 基因的 D1/D2 区有 2.5%（14 个核苷酸取代）的差异，在 ITS 区有 8.0%（40 个核苷酸取代）的差异。基于 ITS 区和 D1/D2 区联合序列的系统发育分析结果表明，DMKU-XD44 代表 （，，，和）分支中的一员，与该分支中其他种属的酵母种在系统发育上是不同的。因此，DMKU-XD44 代表一个新型的酵母属种。提议采用的名称为 f.a.，sp. nov.。其模式种为 DMKU-XD44，而其 MycoBank 编号为 MB 832816。
