Bianchi C, Gutkowska J, Garcia R, Thibault G, Genest J, Cantin M
J Histochem Cytochem. 1987 Feb;35(2):149-53. doi: 10.1177/35.2.3025291.
Using light and electron microscope autoradiography in vivo, the localization of 125I-(Arg 101-Tyr 126) atrial natriuretic factor (ANF)-binding sites was studied in the renal medulla of rats. At the light microscopic level, the autoradiographic reaction was mainly distributed in patches in the outer medulla, and followed the tubular architecture in the innermost part of the inner medulla. At the electron microscopic level, binding sites were mainly found in the outer medullary descending vasa recta and inner medullary collecting ducts. These results suggest that, in rats, the renal medulla may participate in the natriuresis and diuresis produced by ANF through vascular and tubular effects; the former by changing medullary blood flow at the level of descending vasa recta and the latter by acting on electrolyte and water transport at the level of collecting ducts.
运用体内光镜和电镜放射自显影技术，研究了125I-（精氨酸101-酪氨酸126）心房利钠因子（ANF）结合位点在大鼠肾髓质中的定位。在光镜水平，放射自显影反应主要分布在外髓质的斑块中，并在内髓质最内层沿肾小管结构分布。在电镜水平，结合位点主要发现于外髓质直小血管降支和内髓质集合管。这些结果表明，在大鼠中，肾髓质可能通过血管和肾小管效应参与ANF产生的利钠和利尿作用；前者通过改变直小血管降支水平的髓质血流，后者通过作用于集合管水平的电解质和水转运。
