Soligo D, de harven E P
J Histochem Cytochem. 1987 Feb;35(2):267-9. doi: 10.1177/35.2.3025295.
We describe a method to label circulating human granulocytes with an immunogold marker and then incubate them for demonstration of myeloperoxidase activity. The cells were observed in the backscattered electron imaging (BEI) mode of the scanning electron microscope. This permits simultaneous visualization of cell surface morphology, the immunological surface marker, and the cytochemical reactivity of each individual cell. Enhanced identification of the various cell types and more precise characterization of cell surface features in the different steps of leukocyte differentiation are expected to result from application of this technique.
我们描述了一种用免疫金标记物标记循环中的人类粒细胞，然后对其进行孵育以显示髓过氧化物酶活性的方法。在扫描电子显微镜的背散射电子成像（BEI）模式下观察细胞。这允许同时可视化每个细胞的表面形态、免疫表面标记物和细胞化学反应性。应用该技术有望在白细胞分化的不同步骤中增强对各种细胞类型的识别，并更精确地表征细胞表面特征。
