de Harven E
Department of Pathology, Banting Institute, Toronto, Canada.
Ultrastruct Pathol. 1987;11(5-6):711-21. doi: 10.3109/01913128709048458.
Elements of high atomic number backscatter electrons that carry information of cytochemical and of immunocytochemical significance in high resolution scanning electron microscopy (SEM). Reaction products of enzyme cytochemistry containing lead or osmium have been used to localize the sites of phosphatase and of endogenous peroxidase, respectively. Particles of colloidal gold, ranging in diameter from 40 to 13 nm, have been successfully used to label cell surface antigens specifically identified by murine monoclonal antibodies. The diagnostic potential of the scanning electron microscope in clinical hematology appears to be considerably enhanced by the use of the backscattered electron imaging (BEI) mode. Quantitation of the number of gold-labeled epitopes on cell surfaces cannot be achieved in the conventional secondary electron mode of the SEM, but is an attractive possibility in the backscattered electron imaging mode.
在高分辨率扫描电子显微镜（SEM）中，高原子序数的背散射电子携带细胞化学和免疫细胞化学意义的信息。含铅或锇的酶细胞化学反应产物分别用于定位磷酸酶和内源性过氧化物酶的位点。直径范围为40至13纳米的胶体金颗粒已成功用于标记由鼠单克隆抗体特异性识别的细胞表面抗原。通过使用背散射电子成像（BEI）模式，扫描电子显微镜在临床血液学中的诊断潜力似乎得到了显著增强。在SEM的传统二次电子模式下无法实现细胞表面金标记表位数量的定量，但在背散射电子成像模式下这是一个有吸引力的可能性。
