Minaei Samad, Shahabi Shahram, Seyyedi Shahram, Ziaali Naser, Mohammadzadeh Hajipirloo Habib
Department of Parasitology and Mycology, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Department of Microbiology, Immunology and Genetics, Faculty of Medicine, Urmia University of Medical Sciences, Urmia, Iran.
Exp Parasitol. 2018 Nov;194:60-66. doi: 10.1016/j.exppara.2018.09.014. Epub 2018 Sep 22.
Severe or lethal damages, caused by Toxoplasma gondii infection in congenital cases and immunocompromised patients implies the necessity for development of a vaccine and an appropriate adjuvant would be needed to elicit a protective Th1 biased-immune response. The adjuvant activity of propranolol was surveyed and compared with alum by immunization of BALB/c mice with protein components of T. gondii tachyzoites. Five groups of BALB/c mice were immunized with phosphate buffered saline (negative control), Toxoplasma lysate antigen (TLA), alum plus TLA, Propranolol plus TLA, and alum, propranolol and TLA. Immunization efficacy was evaluated by lymphocyte proliferation and DTH tests, challenge with live tachyzoites, IFN-γ production by spleen cells, serum TNF-α concentration and anti- Toxoplasma total IgG, IgG1 and IgG2a measurements. Mice of the PRP-TLA group induced significantly more IFN-γ and TNF-α production and lymphocyte proliferation than other groups. This group of mice also showed more anti-T. gondii IgG2a and DTH responses and showed a significantly increased survival time after challenge. These findings indicate that propranolol as an adjuvant in combination with TLA, may enhance cellular immunity against T. gondii.
在先天性病例和免疫功能低下患者中，由弓形虫感染引起的严重或致命损害意味着开发疫苗的必要性，并且需要一种合适的佐剂来引发偏向Th1的保护性免疫反应。通过用弓形虫速殖子的蛋白质成分免疫BALB/c小鼠，研究了普萘洛尔的佐剂活性并与明矾进行了比较。五组BALB/c小鼠分别用磷酸盐缓冲盐水（阴性对照）、弓形虫裂解物抗原（TLA）、明矾加TLA、普萘洛尔加TLA以及明矾、普萘洛尔和TLA进行免疫。通过淋巴细胞增殖和迟发型超敏反应（DTH）试验、用活速殖子攻击、脾细胞产生的干扰素-γ、血清肿瘤坏死因子-α浓度以及抗弓形虫总IgG、IgG1和IgG2a测量来评估免疫效果。PRP-TLA组小鼠诱导产生的干扰素-γ和肿瘤坏死因子-α以及淋巴细胞增殖明显多于其他组。该组小鼠还表现出更多的抗弓形虫IgG2a和DTH反应，并且在攻击后存活时间显著延长。这些发现表明，普萘洛尔作为佐剂与TLA联合使用，可能增强针对弓形虫的细胞免疫。
