Tappeh Khosrow Hazrati, Khorshidvand Zohre, Shahabi Shahram, Mohammadzadeh Habib
Cellular and Molecular Research Center, Urmia University of Medical Sciences, Urmia, Iran.
Turkiye Parazitol Derg. 2013;37(2):92-6. doi: 10.5152/tpd.2013.22.
The excreted-secreted antigens (ESA) from the tachyzoites seem to play a key role in immunity against Toxoplasma gondii. The aim of this study is to investigate whether Alum-NLT mixture, as a new adjuvant, can induce humoral immunity in response to excreted secreted antigens (ESA) of Toxoplasma gondii as a model vaccine or not.
Six- to eight-week-old female Balb/c mice were divided into five groups. Mice in the experimental groups received either ESA vaccine alone or in combination with the adjuvant Alum, NLT or Alum-NLT mixture; Mice in the negative control group received phosphate buffered saline (PBS). All mice were immunised, three times subcutaneously (s.c.) with a total volume of 150μl each with a 10-day interval. Ten days after the final immunisation, immune response to Toxoplasma gondii was assessed.
Our results revealed that Alum-NLT mixture as an adjuvant during vaccination boosts the efficacy of the ESA vaccine by means of increasing Toxoplasma gondii-specific IgG, IgG2a production and the ratio of IgG2a/IgG1 (P-value < 0.05). The use of this adjuvant mixture improved the protective immunity against Toxoplasma gondii.
Administration of the Alum-NLT mixture as an adjuvant in ESA vaccine enhances humoral immunity.
速殖子分泌排泄抗原（ESA）似乎在抗刚地弓形虫免疫中起关键作用。本研究旨在调查作为新型佐剂的明矾-NLT混合物能否作为模型疫苗诱导针对刚地弓形虫分泌排泄抗原（ESA）的体液免疫。
将6至8周龄雌性Balb/c小鼠分为五组。实验组小鼠分别单独接种ESA疫苗或与佐剂明矾、NLT或明矾-NLT混合物联合接种；阴性对照组小鼠接受磷酸盐缓冲盐水（PBS）。所有小鼠均皮下注射免疫三次，每次总体积150μl，间隔10天。末次免疫后10天，评估对刚地弓形虫的免疫反应。
我们的结果显示，接种疫苗期间使用明矾-NLT混合物作为佐剂，通过增加刚地弓形虫特异性IgG、IgG2a产生以及IgG2a/IgG1比值，提高了ESA疫苗的效力（P值<0.05）。使用这种佐剂混合物改善了对刚地弓形虫的保护性免疫。
在ESA疫苗中使用明矾-NLT混合物作为佐剂可增强体液免疫。
