Organic Polymer Chemistry Research Laboratory, Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Islamic Republic of Iran; Research Institute for Nanotechnology and Advanced Materials, Isfahan University of Technology, Isfahan 84156-83111, Islamic Republic of Iran.
Organic Polymer Chemistry Research Laboratory, Department of Chemistry, Isfahan University of Technology, Isfahan 84156-83111, Islamic Republic of Iran.
Int J Biol Macromol. 2018 Dec;120(Pt B):1451-1460. doi: 10.1016/j.ijbiomac.2018.09.127. Epub 2018 Sep 22.
Crosslinked Chitosan/nitrogen doped-graphene quantum dot nanocomposites (CCS/NGQD NC) with 10 wt% of glutaraldehyd as crosslinker were fabricated under ultrasonic cavitation. Structure and physicochemical properties of the prepared nanocomposites (NC) were systematically characterized by a number of techniques. The swelling capacity of the crosslinked films in the simulated intestinal (pH 7.4) media was evaluated. However, rate of water ingress of specimens was nearly same, but, the CCS/NGQD NC 8 wt% showed the more water absorption ability than the CCS. Bioactivity of the CCS and CCS/NGQD NCs were evaluated by studying their ability to form of apatite on their surface after soaking in a simulated body fluid (SBF) for 30 days. In images of field emission scan electron microscopy, small island-like agglomerated apatite deposited on the sample surface. Also, the pH fluctuation displayed that the hydroxyapatite was formed on the surface of the samples after one month of immersion in SBF. The finding showed that the CCS/NGQD NCs immersed into SBF had a better apatite-forming bioactivity compared with the CCS, which may be due to the high surface area, adequate function groups and appropriate swelling of the NCs. Such improved behaviors of CCS/NGQD NCs may have potential use in the bone tissue engineering.
采用超声空化法制备了壳聚糖/氮掺杂石墨烯量子点纳米复合材料（CCS/NGQD NC），其中戊二醛作为交联剂的含量为 10wt%。通过多种技术对所制备的纳米复合材料（NC）的结构和物理化学性质进行了系统表征。评估了交联膜在模拟肠道（pH 7.4）介质中的溶胀能力。然而，样品的吸水率几乎相同，但 CCS/NGQD NC 8wt%的吸水能力比 CCS 更强。通过研究它们在模拟体液（SBF）中浸泡 30 天后在表面形成磷灰石的能力来评估 CCS 和 CCS/NGQD NC 的生物活性。在场发射扫描电子显微镜的图像中，在样品表面上沉积了小的岛状聚集的磷灰石。此外，pH 波动表明，在 SBF 浸泡一个月后，样品表面形成了羟基磷灰石。研究结果表明，与 CCS 相比，浸泡在 SBF 中的 CCS/NGQD NC 具有更好的形成磷灰石的生物活性，这可能是由于 NCs 的高比表面积、充足的功能基团和适当的溶胀。CCS/NGQD NC 的这种改进行为可能在骨组织工程中有潜在的应用。
