Department of Epidemiology and Biostatistics, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Wuhan Children's Hospital (Wuhan Maternal and Child Healthcare Hospital), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, AState Key Laboratory of Environmental Health (incubating), Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China.
Environ Pollut. 2018 Dec;243(Pt B):1126-1133. doi: 10.1016/j.envpol.2018.09.083. Epub 2018 Sep 19.
Prenatal disinfection by-products (DBPs) exposure is linked with adverse birth outcomes. Genetic susceptibility to DBP metabolism may modify the exposure-outcome associations.
To investigate whether CYP2E1 and GSTZ1 genetic polymorphisms modified the associations of prenatal DBP exposures with adverse birth outcomes.
Two biomarkers of DBP exposures including trihalomethanes (THMs) in blood and trichloroacetic acid (TCAA) in urine were determined among 426 pregnant women from a Chinese cohort study. CYP2E1 (rs2031920, rs3813867, and rs915906) and GSTZ1 (rs7975) polymorphisms in cord blood were genotyped. Statistical interactions between prenatal DBP exposures and newborns CYP2E1 and GSTZ1 polymorphisms on birth outcomes (birth weight, birth length, and gestational age) were examined by multivariable linear regression with adjustment for potential confounders.
We found that newborns CYP2E1 genetic polymorphisms (rs2031920 and rs3813867) modified the associations of maternal blood THMs or urinary TCAA levels with birth outcomes. However, these interactions were nonsignificant after Bonferroni correction for multiple comparisons, except for the interaction between maternal blood BrTHMs [sum of dibromochloromethane (DBCM), bromodichloromethane (BDCM), and bromoform (TBM)] and newborns CYP2E1 gene rs2031920 polymorphisms on birth weight (P for interaction = 0.003).
Newborns genetic variations of CYP2E1 rs2031920 may modify the impacts of prenatal BrTHM exposure on birth weight. This finding needs to be further confirmed.
产前消毒副产物（DBPs）暴露与不良出生结局有关。DBP 代谢的遗传易感性可能会改变暴露-结局关联。
研究 CYP2E1 和 GSTZ1 基因多态性是否改变了产前 DBP 暴露与不良出生结局的关联。
在中国队列研究中，对 426 名孕妇的两种 DBP 暴露生物标志物（血液中的三卤甲烷（THMs）和尿液中的三氯乙酸（TCAA））进行了测定。在脐带血中检测了 CYP2E1（rs2031920、rs3813867 和 rs915906）和 GSTZ1（rs7975）多态性。通过多变量线性回归，调整潜在混杂因素后，检查了产前 DBP 暴露与新生儿 CYP2E1 和 GSTZ1 多态性对出生结局（出生体重、出生体长和胎龄）的统计学交互作用。
我们发现，新生儿 CYP2E1 遗传多态性（rs2031920 和 rs3813867）改变了母体血液 THMs 或尿液 TCAA 水平与出生结局的关系。然而，在对多次比较进行 Bonferroni 校正后，这些相互作用并不显著，除了母体血液 BrTHMs（二溴一氯甲烷（DBCM）、溴二氯甲烷（BDCM）和溴仿（TBM）之和）与新生儿 CYP2E1 基因 rs2031920 多态性之间对出生体重的相互作用（P 交互作用=0.003）。
新生儿 CYP2E1 rs2031920 的遗传变异可能改变了产前 BrTHM 暴露对出生体重的影响。这一发现需要进一步证实。
