Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, 266100 Qingdao, China.
Key Lab of Marine Environment and Ecology, Ministry of Education, Ocean University of China, 266100 Qingdao, China; College of Environmental Science and Engineering, Ocean University of China, 266100 Qingdao, China.
Sci Total Environ. 2019 Feb 15;651(Pt 1):859-870. doi: 10.1016/j.scitotenv.2018.09.218. Epub 2018 Sep 18.
In this study, the simultaneous partial nitrification and denitrification (SPND) process was investigated in a hybrid sequencing batch biofilm reactor (HSBBR) fed with synthetic wastewater with 1.2% salinity. Different anaerobic/aerobic (An/Ae) durations were selected for evaluating the removal performance of contaminants and the succession of the microbial community in the reactor. The highest organic matter removal efficiency was obtained at An/Ae hour ratio of 0/6.5, with an average chemical oxygen demand (COD) removal of 89.6% at the steady state. Similarly high nitrogen removal efficiencies were achieved at An/Ae hour ratios of 1/5.5, 1.5/5 and 2/4.5,with over 92% of average total nitrogen removed. This represents an increase of more than 10% compared to the mode with An/Ae hour ratio of 0/6.5. High-throughput sequencing analysis revealed that the increase of the An/Ae hour ratio changed the characteristics of the community structures in the HSBBR. Azoarcus was the most dominant genus when the An/Ae hour ratio was 0/6.5 in both suspended sludge (S-sludge) and biofilm, while Candidatus_Competibacter was the most abundant genus at An/Ae hour ratios of 2/4.5 and 3/3.5. Nitrosomonas was the only ammonia oxidizing bacteria (AOB) detected in this study. Nitrospira, a kind of nitrite oxidizing bacteria (NOB), was sensitive to salinity and altering the An/Ae mode; this was detected only in S-sludge samples in a fully aerobic mode with a low percentage of 0.1%. S-sludge and biofilm samples shared a similar bacterial composition. This research demonstrated that efficient nitrogen and carbon removal could be achieved via the SPND process by the symbiotic functional groups in a hybrid S-sludge and biofilm reactor.
在这项研究中，采用 1.2%盐度的合成废水，在混合序批式生物膜反应器（HSBBR）中考察了同时硝化反硝化（SPND）过程。选择不同的厌氧/好氧（An/Ae）时间来评估反应器中污染物的去除性能和微生物群落的演替。在 An/Ae 小时比为 0/6.5 时，获得了最高的有机物去除效率，在稳定状态下平均化学需氧量（COD）去除率为 89.6%。同样，在 An/Ae 小时比为 1/5.5、1.5/5 和 2/4.5 时，实现了高氮去除效率，平均总氮去除率超过 92%。与 An/Ae 小时比为 0/6.5 的模式相比，这增加了 10%以上。高通量测序分析表明，An/Ae 小时比的增加改变了 HSBBR 中群落结构的特征。当 An/Ae 小时比为 0/6.5 时，悬浮污泥（S-污泥）和生物膜中最优势的属是 Azorarcus，而在 An/Ae 小时比为 2/4.5 和 3/3.5 时，最丰富的属是 Candidatus_Competibacter。在本研究中，检测到的唯一氨氧化菌（AOB）是 Nitrosomonas。亚硝酸盐氧化菌（NOB）Nitrospira 对盐度敏感，改变 An/Ae 模式；仅在完全好氧模式下的 S-污泥样品中检测到，比例低至 0.1%。S-污泥和生物膜样品具有相似的细菌组成。本研究表明，通过混合 S-污泥和生物膜反应器中的共生功能群，可以通过 SPND 过程实现高效的氮和碳去除。
