Key Laboratory of Building Safety and Energy Efficiency, Ministry of Education, Department of Water Engineering and Science, College of Civil Engineering, Hunan University, Changsha, 410082, China.
School of Civil Engineering, Wuhan University, Wuhan, 430072, China.
J Hazard Mater. 2020 May 15;390:121782. doi: 10.1016/j.jhazmat.2019.121782. Epub 2019 Dec 6.
Hypersaline wastewater may pose threats to biological wastewater treatment processes. An aerobic granular sludge-based sequencing batch reactor (SBR) performing simultaneous nitrification, denitrification and phosphorus removal (SNDPR) was evaluated with increased salinity from 1 to 2 % (w/v). Nitrogen removal performance was unaffected by salinity up to 20 g/L in terms of reliable and efficient nitrification and denitrification. Enhanced biological phosphorus removal (EBPR) process was completely deteriorated at salinity up to 2 %, in contrast to excellent phosphorus removal at 1 %. Profiles of phosphorus over one cycle demonstrated that higher salinity not only inhibited anaerobic phosphorus release but also impeded aerobic/anoxic phosphorus uptake. Illumina MiSeq sequencing revealed multiple halophilic and non-halophilic bacteria within aerobic granules with family Anaerolineaceae being the predominant potential salt adapter. Besides, ammonia oxidizing bacteria (AOB), glycogen accumulating organisms (GAOs) were more tolerant to salt than nitrite oxidizing bacteria (NOB) and phosphorus accumulating organisms (PAOs) and denitrifying PAOs (DNPAOs). These results deciphered the resilience of aerobic granular sludge-based biological nitrogen and phosphorus removal processes to hypersaline stress.
高盐废水可能对生物废水处理过程造成威胁。采用基于好氧颗粒污泥的序批式反应器（SBR）进行同步硝化反硝化除磷（SNDPR），评估了从 1%到 2%（w/v）增加盐度的情况。在 20g/L 的盐度下，氮的去除性能不受影响，实现了可靠和高效的硝化和反硝化。相比之下，在 2%的盐度下，增强型生物除磷（EBPR）过程完全恶化，而在 1%的盐度下则具有良好的除磷效果。一个周期内的磷分布表明，较高的盐度不仅抑制了厌氧释磷，还阻碍了好氧/缺氧吸磷。Illumina MiSeq 测序揭示了好氧颗粒内存在多种嗜盐菌和非嗜盐菌，其中产甲烷菌科是主要的潜在盐适应菌。此外，氨氧化细菌（AOB）和糖原积累菌（GAOs）比亚硝酸盐氧化细菌（NOB）和磷积累菌（PAOs）以及反硝化除磷菌（DNPAOs）更能耐受盐度。这些结果揭示了好氧颗粒污泥基生物脱氮除磷过程对高盐胁迫的恢复能力。
