Rae B P, Elliott R M
J Gen Virol. 1986 Dec;67 ( Pt 12):2635-43. doi: 10.1099/0022-1317-67-12-2635.
Temperature-sensitive (ts) mutants of vesicular stomatitis virus, New Jersey serotype, classified in complementation group E contain lesions in the NS gene, which manifest as marked electrophoretic mobility differences of the mutant NS proteins in SDS-polyacrylamide gels. We have cloned full-length cDNA copies of the mutant NS mRNAs, and have determined their nucleotide sequences. tsE1 and tsE3 had single nucleotide changes, and tsE2 had two nucleotide changes, compared to the wild-type NS gene. Three of the mutations were clustered in a region of 18 nucleotides. All the nucleotide differences resulted in amino acid substitutions, which in each case changed the charge of the amino acid concerned. Analysis of the wild-type and mutant NS protein sequences by the method of Chou & Fasman indicated that single amino acid substitutions can radically alter the predicted secondary structure, and these data are discussed in relation to the observed electrophoretic mobility differences.
新泽西血清型水泡性口炎病毒的温度敏感（ts）突变体，归类于互补群E，其NS基因存在损伤，这表现为突变型NS蛋白在SDS-聚丙烯酰胺凝胶中的显著电泳迁移率差异。我们克隆了突变型NS mRNA的全长cDNA拷贝，并确定了它们的核苷酸序列。与野生型NS基因相比，tsE1和tsE3有单个核苷酸变化，tsE2有两个核苷酸变化。其中三个突变聚集在18个核苷酸的区域内。所有核苷酸差异都导致了氨基酸替换，每种情况下都改变了相关氨基酸的电荷。用Chou和Fasman的方法对野生型和突变型NS蛋白序列进行分析表明，单个氨基酸替换可从根本上改变预测的二级结构，并且结合观察到的电泳迁移率差异对这些数据进行了讨论。
