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蛋白激酶C在前叶垂体肿瘤细胞中的易位

Translocation of protein kinase C in anterior pituitary tumor cells.

作者信息

Zatz M, Mahan L C, Reisine T

出版信息

J Neurochem. 1987 Jan;48(1):106-10. doi: 10.1111/j.1471-4159.1987.tb13133.x.

Abstract

Previous studies have shown that phorbol esters and lithium each stimulate the secretion of adrenocorticotropic hormone (ACTH) by the anterior pituitary tumor cell line AtT20/D16-16. Pretreatment with either lithium or phorbol ester desensitizes the cells to subsequent stimulation by phorbol ester. An early consequence of phorbol ester action in other systems is the translocation of protein kinase C from cytosol to membranes. We have assayed protein kinase C activity in cytosol and membranes of AtT20 cells after treatment with phorbol dibutyrate, lithium, or other agents that stimulate secretion of ACTH in these cells. Phorbol dibutyrate clearly induced translocation of protein kinase C, but lithium treatment did not cause translocation itself, nor did pretreatment with lithium affect the translocation induced by phorbol dibutyrate. These results are consistent with a role for translocation of protein kinase C in the stimulatory and desensitizing effects of phorbol esters but fail to implicate translocation in the actions of lithium on AtT20 cells.

摘要

先前的研究表明,佛波酯和锂各自都能刺激垂体前叶肿瘤细胞系AtT20/D16-16分泌促肾上腺皮质激素(ACTH)。用锂或佛波酯预处理会使细胞对随后佛波酯的刺激产生脱敏作用。在其他系统中,佛波酯作用的一个早期结果是蛋白激酶C从胞质溶胶转位至细胞膜。在用二丁酰佛波酯、锂或其他能刺激这些细胞分泌ACTH的试剂处理后,我们测定了AtT20细胞胞质溶胶和细胞膜中的蛋白激酶C活性。二丁酰佛波酯明显诱导了蛋白激酶C的转位,但锂处理本身并未导致转位,用锂预处理也不影响二丁酰佛波酯诱导的转位。这些结果与蛋白激酶C转位在佛波酯的刺激和脱敏作用中所起的作用一致,但未能表明转位与锂对AtT20细胞的作用有关。

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