定向 sortase A 进化用于在有机共溶剂中高效的定点生物偶联。
Directed sortase A evolution for efficient site-specific bioconjugations in organic co-solvents.
机构信息
DWI - Leibniz Institute for Interactive Materials, Forckenbeckstraβe 50, 52056 Aachen, Germany.
出版信息
Chem Commun (Camb). 2018 Oct 9;54(81):11467-11470. doi: 10.1039/c8cc06017g.
Directed sortase A evolution yielded the variants R159G and D165Q/D186G/K196V with increased resistance (2.2-fold) and catalytic efficiency (6.3-fold) in 45% (v/v) dimethylsulfoxide. Interestingly, D165Q/D186G/K196V also showed an up to 4.7-fold increased activity for the conjugation of hydrophobic peptides/amines in co-solvents. MD simulations revealed that conformational mobilities are important for the gained resistance.
定向的 sortase A 进化产生了变体 R159G 和 D165Q/D186G/K196V，它们在 45%（v/v）二甲基亚砜中的抗性（提高了 2.2 倍）和催化效率（提高了 6.3 倍）。有趣的是，D165Q/D186G/K196V 对共溶剂中疏水性肽/胺的缀合反应的活性也提高了 4.7 倍。MD 模拟表明，构象迁移率对于获得的抗性很重要。
Zotero 插件