Bishop D K, Kolodner R D
Mol Cell Biol. 1986 Oct;6(10):3401-9. doi: 10.1128/mcb.6.10.3401-3409.1986.
Purified heteroduplex plasmid DNAs containing 8- or 12-base-pair insertion mismatches or AC or CT substitution mismatches were used to transform Saccharomyces cerevisiae. Two insertion mismatches, separated by 943 base pairs, were repaired independently of each other at least 55% of the time. This suggested that repair tracts were frequently shorter than 1 kilobase. The two insertion mismatches were repaired with different efficiencies. Comparison of the repair efficiency of one mismatched site with or without an adjacent mismatch suggests that mismatches promote their own repair and can influence the repair of neighboring mismatches. When two different plasmids containing single-insertion mismatches were transformed into S. cerevisiae cells, a slight preference towards insertion was detected among repair products of one of the two plasmids, while no repair preference was detected among transformants with the second plasmid.
含有8或1个12个碱基对插入错配或AC或CT替换错配的纯化异源双链体质粒DNA被用于转化酿酒酵母。两个由943个碱基对隔开的插入错配，至少55%的时间里彼此独立修复。这表明修复片段通常短于1千碱基。两个插入错配的修复效率不同。比较一个错配位点在有或没有相邻错配情况下的修复效率表明，错配促进自身修复并能影响相邻错配的修复。当将两个含有单插入错配的不同质粒转化到酿酒酵母细胞中时，在两个质粒之一的修复产物中检测到对插入的轻微偏好，而在第二个质粒的转化体中未检测到修复偏好。
