Canecki-Varžić Silvija, Prpić-Križevac Ivana, Mihaljević Silvio, Bilić-Ćurčić Ines, Alkhamis Tamara, Wagner Jasenka, Škrlec Ivana, Barbić Jerko
Department of Diabetes, Endocrinology and Metabolism Disorders, Osijek University Hospital Centre, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia.
Department of Gastroenterology and Hepatology, Osijek University Hospital Centre, Faculty of Medicine, Josip Juraj Strossmayer University of Osijek, Osijek, Croatia.
Acta Clin Croat. 2018 Mar;57(1):71-81. doi: 10.20471/acc.2018.57.01.08.
Interleukin (IL)-10 is an anti-inflammatory cytokine, and a decrease in its secretion is associated with obesity, metabolic syndrome and type 2 diabetes. However, it has not been established whether the intensity of the immune response during diabetes-associated chronic inflammation affects the development and/or progression of type 2 diabetes and its microvascular complications. The aim of this study was to investigate the role of single nucleotide polymorphism (SNP)-1082G/A for IL-10 gene in development of diabetes type 2 and its complications. DNA was extracted from blood cells of 240 overweight/obese subjects for IL-10 genotyping. Based on the presence of diabetes type 2, patients were divided in two groups: experimental group of 144 patients with diabetes type 2 and control group of 96 age- and gender-matched subjects without diabetes. Compared to control group, diabetic group had higher levels of leukocytes (p=0.012), fibrinogen (p=0.049) and plasminogen activator inhibitor-1 (PAI-1) (p=0.009), and lower levels of albumin (p=0.001). There were no differences in the frequency of SNP-1082G/A for IL-10 gene between the two groups (p=0.654). When considering diabetes related traits in all subjects in relation to specific genotype, a group with homozygous (AA) genotype had higher values of the mean fasting glucose (p<0.000001), HbA1c (p<0.000001) and HOMA-IR (p=0.003632), while the mean HOMA-B value (p=0.000178) was lower when compared to the groups with GG and GA genotypes. There was no difference in devel-opment of diabetic nephropathy, retinopathy and polyneuropathy between the IL-10 polymorphism genotypes. In conclusion, obese diabetes type 2 patients had an increased inflammation activity com-pared to obese non-diabetic individuals. There was no association of the investigated polymorphisms and development of type 2 diabetes and its microvascular complications. However, diabetes related traits clearly depended on the presence of specific IL-10 genotype.
白细胞介素（IL）-10是一种抗炎细胞因子，其分泌减少与肥胖、代谢综合征和2型糖尿病相关。然而，糖尿病相关慢性炎症期间免疫反应的强度是否会影响2型糖尿病及其微血管并发症的发生和/或进展尚未明确。本研究的目的是调查白细胞介素-10基因单核苷酸多态性（SNP）-1082G/A在2型糖尿病及其并发症发生中的作用。从240名超重/肥胖受试者的血细胞中提取DNA进行白细胞介素-10基因分型。根据是否患有2型糖尿病，将患者分为两组：144例2型糖尿病患者的实验组和96例年龄及性别匹配的非糖尿病受试者的对照组。与对照组相比，糖尿病组白细胞（p = 0.012）、纤维蛋白原（p = 0.049）和纤溶酶原激活物抑制剂-1（PAI-1）（p = 0.009）水平较高，白蛋白水平较低（p = 0.001）。两组间白细胞介素-10基因SNP-1082G/A的频率无差异（p = 0.654）。当考虑所有受试者中与特定基因型相关的糖尿病相关特征时，纯合子（AA）基因型组的平均空腹血糖（p < 0.000001）、糖化血红蛋白（HbA1c）（p < 0.000001）和胰岛素抵抗指数（HOMA-IR）（p = 0.003632）值较高，而与GG和GA基因型组相比，平均胰岛β细胞功能指数（HOMA-B）值较低（p = 0.000178）。白细胞介素-10基因多态性基因型之间糖尿病肾病、视网膜病变和多发性神经病变的发生率无差异。总之，与肥胖非糖尿病个体相比，肥胖2型糖尿病患者的炎症活动增加。所研究的多态性与2型糖尿病及其微血管并发症的发生无关联。然而，糖尿病相关特征明显取决于特定白细胞介素-10基因型的存在。
