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MES-1小鼠增强子元件与两个不同转录单元的异质性5'末端密切相关。

The MES-1 murine enhancer element is closely associated with the heterogeneous 5' ends of two divergent transcription units.

作者信息

Williams T J, Fried M

出版信息

Mol Cell Biol. 1986 Dec;6(12):4558-69. doi: 10.1128/mcb.6.12.4558-4569.1986.

DOI:10.1128/mcb.6.12.4558-4569.1986
PMID:3025660
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC367240/
Abstract

The location in the mouse genome of the 149-base pair MES-1 element, previously isolated by its ability to restore expression to an enhancerless selectable gene, was analyzed. The active moiety of the single-copy MES-1 element is located between the 5' ends of two divergent transcription units, SURF-1 and SURF-2, both of which specify more than one mRNA species by differential splicing. The heterogeneous 5' ends of the SURF transcripts are separated by only 50 to 75 base pairs, and this sequence possesses a high G + C content (65%) and contains neither the TATA and CAAT box motifs normally associated with many highly expressed genes nor the GC box motif (Sp1-binding site) associated with a number of housekeeping genes. Although MES-1 appears to have enhancerlike properties when linked to heterologous genes, its normal genomic location suggests that it functions as a bidirectional promoter. Thus, MES-1 may represent a new class of enhancer-promoter element.

摘要

对149个碱基对的MES-1元件在小鼠基因组中的位置进行了分析，该元件先前因其能恢复无增强子的可选择基因的表达能力而被分离出来。单拷贝MES-1元件的活性部分位于两个反向转录单元SURF-1和SURF-2的5'端之间，这两个转录单元都通过可变剪接产生不止一种mRNA。SURF转录本的异质5'端仅相隔50至75个碱基对，该序列具有较高的G + C含量（65%），既不包含通常与许多高表达基因相关的TATA和CAAT框基序，也不包含与一些管家基因相关的GC框基序（Sp1结合位点）。尽管MES-1与异源基因相连时似乎具有类似增强子的特性，但其正常的基因组位置表明它作为双向启动子发挥作用。因此，MES-1可能代表了一类新型的增强子-启动子元件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e6a/367240/1c665253f4da/molcellb00096-0432-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e6a/367240/cbda92955cf2/molcellb00096-0425-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e6a/367240/30cc5b91760b/molcellb00096-0429-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e6a/367240/1c665253f4da/molcellb00096-0432-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e6a/367240/cbda92955cf2/molcellb00096-0425-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e6a/367240/30cc5b91760b/molcellb00096-0429-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e6a/367240/1c665253f4da/molcellb00096-0432-a.jpg

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