Xu Feng, Sang Wentao, Li Ling, He Xinyu, Wang Feng, Wen Taoqun, Zeng Nan
a Department of Pharmacology, College of Pharmacy , Chengdu University of TCM , Chengdu , Sichuan , P.R. China.
Drug Chem Toxicol. 2019 May;42(3):309-316. doi: 10.1080/01480545.2018.1509987. Epub 2018 Sep 27.
Rimulus cinnamon is the dried twig of Cinnamomum cassia Presl. It is widely used in China for the treatment of inflammatory processes, amenorrhea, and other diseases. We aimed to study the protective effects of ethyl acetate extracts of R. cinnamon (EAE) on systemic inflammation and lung injury in endotoxin-poisoned mice. EAE was administered 5 d prior to lipopolysaccharide (LPS) challenge with 15 mg/kg LPS. The administration of EAE increased the levels of interferon-γ (IFN-γ) and decreased the levels of interleukin-18 (IL-18) and tumor necrosis factor-α (TNF-α) in the serum. Additionally, EAE relieved the pathological changes in the tissues of the lungs and spleen, and significantly reduced the number of neutrophils in the lung tissues. In addition, treatment with EAE decreased the mRNA expression of the NLR family, pyrin domain-containing protein 3 (NLRP3), caspase-1, and interleukin-1β (IL-1β) in the lungs, as well as the expression of NLRP3, caspase-1 (p20), and pro-IL-1β proteins. These results demonstrated the promising anti-inflammatory effects of EAE in endotoxin-poisoned mice. Furthermore, EAE could alleviate the lung injury of endotoxin-poisoned mice by antagonizing the activation of the NLRP3 inflammasome.
桂枝为樟科植物肉桂的干燥嫩枝。在中国，它被广泛用于治疗炎症、闭经及其他疾病。我们旨在研究桂枝乙酸乙酯提取物（EAE）对内毒素中毒小鼠全身炎症和肺损伤的保护作用。在用15 mg/kg脂多糖（LPS）攻击前5天给予EAE。给予EAE可提高血清中干扰素-γ（IFN-γ）水平，并降低白细胞介素-18（IL-18）和肿瘤坏死因子-α（TNF-α）水平。此外，EAE减轻了肺和脾组织的病理变化，并显著减少了肺组织中的中性粒细胞数量。此外，EAE处理降低了肺中NLR家族含pyrin结构域蛋白3（NLRP3）、半胱天冬酶-1（caspase-1）和白细胞介素-1β（IL-1β）的mRNA表达，以及NLRP3、半胱天冬酶-1（p20）和前白细胞介素-1β蛋白的表达。这些结果表明EAE在内毒素中毒小鼠中具有良好的抗炎作用。此外，EAE可通过拮抗NLRP3炎性小体的激活来减轻内毒素中毒小鼠的肺损伤。
