Khadrawy Yasser A, El-Gizawy Mayada M, Sorour Safwa M, Sawie Hussein G, Hosny Eman N
a Medical Physiology Department , National Research Centre , Giza , Egypt.
b Department of Pharmacology, Faculty of Medicine , Benha University , Benha , Egypt.
Drug Chem Toxicol. 2019 Mar;42(2):194-202. doi: 10.1080/01480545.2018.1504058. Epub 2018 Sep 27.
The present study is conducted to evaluate the neuroprotective effect of curcumin nanoparticles (CUR NP) against the neurotoxicity induced by cisplatin (CP) in rat. Rats were divided into control group that received saline solution, CP-treated rats that received a single i.p. injection of CP (12 mg/kg body wt), and CP-treated rats that received a single i.p injection of CP (12 mg/kg body wt) followed by a daily oral administration of CUR NP (50 mg/kg body wt) for 14 days. At the end of the experiment, the motor activity of rats was evaluated by open field test. The neurochemical and histopathological changes were investigated in the cerebral cortex. A significant decrease in motor activity was observed in CP-treated rats. This was associated with a significant increase in the cortical levels of lipid peroxidation, nitric oxide, tumor necrosis factor-α, caspase-3, and acetylcholinesterase activity. However, CP induced a significant decrease in reduced glutathione levels and Na, K-ATPase activity. In rats treated with CP and CUR NP, no significant changes were recorded in the parameters of the open field test as compared to control. In addition, treatment with CUR NP prevented all the neurochemical changes induced by CP except the increased value of nitric oxide. CUR NP also reduced the histopathological changes induced by CP. It is clear from the present data that CUR NP could ameliorate the neurotoxic effect induced by cisplatin.
本研究旨在评估姜黄素纳米颗粒（CUR NP）对顺铂（CP）诱导的大鼠神经毒性的神经保护作用。将大鼠分为对照组，给予生理盐水；CP处理组，单次腹腔注射CP（12毫克/千克体重）；CP处理组，单次腹腔注射CP（12毫克/千克体重），随后每日口服CUR NP（50毫克/千克体重）共14天。实验结束时，通过旷场试验评估大鼠的运动活性。在大脑皮层中研究神经化学和组织病理学变化。在CP处理的大鼠中观察到运动活性显著降低。这与皮质脂质过氧化、一氧化氮、肿瘤坏死因子-α、半胱天冬酶-3水平以及乙酰胆碱酯酶活性的显著增加有关。然而，CP导致还原型谷胱甘肽水平和钠钾-ATP酶活性显著降低。在CP和CUR NP处理的大鼠中，与对照组相比，旷场试验参数未记录到显著变化。此外，除一氧化氮值增加外，CUR NP处理可预防CP诱导的所有神经化学变化。CUR NP还减少了CP诱导的组织病理学变化。从目前的数据可以清楚地看出，CUR NP可以改善顺铂诱导的神经毒性作用。
