Biophysics Department, Faculty of Science, Cairo University, Giza, Egypt.
Medical Physiology Department, National Research Centre, Giza, Egypt.
Biochim Biophys Acta Mol Basis Dis. 2020 May 1;1866(5):165665. doi: 10.1016/j.bbadis.2020.165665. Epub 2020 Jan 7.
The present study designed to investigate the protective effect of curcumin nanoparticles (CUR-NPs) on the cardiotoxicity induced by doxorubicin. Rats were divided into four groups; control, rats treated daily with CUR-NPs (50 mg/kg) for 14 days, rats treated with an acute dose of doxorubicin (20 mg/kg) and rats treated daily with CUR-NPs for 14 days injected with doxorubicin on the 10th day. After electrocardiogram (ECG) recording from rats at different groups, rat decapitation was carried out and the heart of each rat was excised out to measure the oxidative stress parameters; lipid peroxidation (MDA), nitric oxide (NO) and reduced glutathione (GSH) and the activities of Na,K,ATPase and acetylcholinesterase (AchE). In addition, the levels of dopamine (DA), norepinephrine (NE) and serotonin (5-HT) were determined in the cardiac tissues. Lactate dehydrogenase (LDH) activity was measured in the serum. The ECG recordings indicated that daily pretreatment with CUR- NPs has prevented the tachycardia (i.e. increase in heart rate) and ameliorated the changes in ST wave and QRS complex induced by doxorubicin. In addition, CUR-NPs prevented doxorubicin induced significant increase in MDA, NO, DA, AchE and LDH and doxorubicin induced significant decrease in GSH, NE, 5-HT and Na,K,ATPase. According to the present findings, it could be concluded that CUR-NPs have a protective effect against cardiotoxicity induced by doxorubicin. This may shed more light on the importance of CUR-NPs pretreatment before the application of doxorubicin therapy.
本研究旨在探讨姜黄素纳米粒子（CUR-NPs）对多柔比星诱导的心脏毒性的保护作用。大鼠分为四组：对照组、连续 14 天每天给予 CUR-NPs（50mg/kg）的大鼠、一次性给予多柔比星（20mg/kg）的大鼠和连续 14 天给予 CUR-NPs 并在第 10 天给予多柔比星的大鼠。在不同组别的大鼠进行心电图（ECG）记录后，大鼠断头并取出心脏，以测量氧化应激参数；脂质过氧化（MDA）、一氧化氮（NO）和还原型谷胱甘肽（GSH）以及 Na,K,ATP 酶和乙酰胆碱酯酶（AchE）的活性。此外，还测定了心脏组织中的多巴胺（DA）、去甲肾上腺素（NE）和 5-羟色胺（5-HT）的水平。测定血清中的乳酸脱氢酶（LDH）活性。心电图记录表明，连续预处理 CUR-NPs 可预防多柔比星引起的心动过速（即心率增加），并改善多柔比星引起的 ST 波和 QRS 复合波的变化。此外，CUR-NPs 可预防多柔比星引起的 MDA、NO、DA、AchE 和 LDH 显著增加，以及多柔比星引起的 GSH、NE、5-HT 和 Na,K,ATP 酶显著减少。根据目前的研究结果，可以得出结论，CUR-NPs 对多柔比星诱导的心脏毒性具有保护作用。这可能更强调了在应用多柔比星治疗前进行 CUR-NPs 预处理的重要性。
