Hau Samantha J, Haan Jisun S, Davies Peter R, Frana Timothy, Nicholson Tracy L
Department of Veterinary Diagnostic and Production Animal Medicine, College of Veterinary Medicine, Iowa State University, Ames, IA, United States.
Enterics Unit-Infectious Disease Lab, Public Health Laboratory Division, Minnesota Department of Health, Saint Paul, MN, United States.
Front Microbiol. 2018 Sep 11;9:2102. doi: 10.3389/fmicb.2018.02102. eCollection 2018.
Antimicrobial resistance (AMR) is an expanding public health concern and methicillin resistant (MRSA) is a notable example. Since the discovery of livestock associated MRSA (LA-MRSA), public health concerns have arisen surrounding the potential of LA-MRSA isolates to serve as a reservoir for AMR determinants. In this study, we compare swine associated LA-MRSA ST5 and human clinical MRSA ST5 isolates for phenotypic antimicrobial susceptibilities determined via broth microdilution and genotypic determinants of AMR using whole genome sequencing and comparative genomic analysis to identify AMR elements. Swine associated LA-MRSA ST5 isolates exhibited phenotypic resistance to fewer antibiotics than clinical MRSA ST5 isolates from humans with no swine contact. Distinct genomic AMR elements were harbored by each subgroup, with little overlap in shared AMR genes between swine associated LA-MRSA ST5 and clinical MRSA ST5 isolates. Our results demonstrate that phenotypic antimicrobial susceptibilities and genotypic determinants of AMR among swine associated LA-MRSA ST5 and clinical MRSA ST5 isolates are separate and distinct.
抗菌药物耐药性（AMR）是一个日益严重的公共卫生问题，耐甲氧西林金黄色葡萄球菌（MRSA）就是一个显著的例子。自发现与家畜相关的MRSA（LA-MRSA）以来，围绕LA-MRSA分离株作为AMR决定因素储存库的可能性引发了公共卫生方面的担忧。在本研究中，我们比较了猪源LA-MRSA ST5分离株和人类临床MRSA ST5分离株的表型抗菌药物敏感性（通过肉汤微量稀释法测定）以及使用全基因组测序和比较基因组分析来鉴定AMR元件的AMR基因型决定因素。猪源LA-MRSA ST5分离株对较少抗生素表现出表型耐药性，相比之下，与猪无接触的人类临床MRSA ST5分离株则不然。每个亚组都含有独特的基因组AMR元件，猪源LA-MRSA ST5和临床MRSA ST5分离株之间共享的AMR基因几乎没有重叠。我们的结果表明，猪源LA-MRSA ST5和临床MRSA ST5分离株之间的表型抗菌药物敏感性和AMR基因型决定因素是相互独立且不同的。
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