Messedi Meriam, Naifar Manel, Grayaa Sahar, Frikha Faten, Messoued Mariem, Sethom Mohamed Marouene, Feki Moncef, Kaabach Naziha, Bahloul Zouheir, Jamoussi Kamel, Ayedi Fatma
Unit of Research Molecular Bases of Human Diseases, 12ES17, Faculty of Medicine of Sfax, University of Sfax, 3029 Sfax, Sfax, Tunisia.
Biochemistry laboratory, Habib Bourguiba University Hospital, 3029 Sfax, Sfax, Tunisia.
Open Rheumatol J. 2018 Aug 31;12:139-151. doi: 10.2174/1874312901812010139. eCollection 2018.
Fatty Acid (FA) composition of serum has been associated with many markers of inflammation. In this study, we tried to examine plasma Saturated Fatty Acid (SFA) and Monounsaturated Fatty Acid (MUFA) composition in Behçet's Disease (BD) patients. The associations between the circulating FA levels and some markers of inflammation have also been investigated.
This study is a cross-sectional one. In fact, a total of 101 BD patients and healthy controls group of 99 subjects are enrolled. Gas Chromatograph equipped with a Capillary Split/Splitless Injector and flame ionization detector was used to analyze the plasma SFA and MUFA compositions. The high sensitivity C-Reactive Protein (hsCRP) and fibrinogen levels were measured using standard techniques.
BD patients had significantly higher proportions of Mystiric Acid (MA), Palmitic Acid (PAM), Palmitoleic Acid (POA) and Stearoyl-CoA Desaturase (SCD)-16, compared to controls.The results revealed that patients with severe involvements had high levels of POA and total MUFA associated with higher SCD-16 activity compared to those with minor ones. The receiver operator characteristic curve analysis revealed that POA could well discriminate BD patients with severe clinical manifestations. In the bivariate analysis, hsCRP was found to be positively correlated with total SAFA and POA elongase activity index but negatively correlated with SCD-18 activity index. The STA, POA, elongase and SCD-16 activity index are correlated with fibrinogen. On the other hand, the multivariate analysis showed that POA remained associated with higher levels of hsCRP.
Unfavourable plasma SFA and MUFA profile were reported in BD patients. POA, which is associated with higher plasma hsCRP level, may play a role in the pathogenesis of BD.
血清脂肪酸（FA）组成与许多炎症标志物相关。在本研究中，我们试图检测白塞病（BD）患者血浆中饱和脂肪酸（SFA）和单不饱和脂肪酸（MUFA）的组成。还研究了循环脂肪酸水平与一些炎症标志物之间的关联。
本研究为横断面研究。实际上，共纳入了101例BD患者和99名健康对照者。使用配备毛细管分流/不分流进样器和火焰离子化检测器的气相色谱仪分析血浆SFA和MUFA组成。采用标准技术测量高敏C反应蛋白（hsCRP）和纤维蛋白原水平。
与对照组相比，BD患者的肉豆蔻酸（MA）、棕榈酸（PAM）、棕榈油酸（POA）和硬脂酰辅酶A去饱和酶（SCD）-16的比例显著更高。结果显示，与轻度受累患者相比，重度受累患者的POA和总MUFA水平较高，且SCD-16活性较高。受试者工作特征曲线分析显示，POA能够很好地区分具有严重临床表现的BD患者。在双变量分析中，发现hsCRP与总饱和脂肪酸（SAFA）和POA延长酶活性指数呈正相关，但与SCD-18活性指数呈负相关。硬脂酸（STA）、POA、延长酶和SCD-16活性指数与纤维蛋白原相关。另一方面，多变量分析显示POA仍与较高水平的hsCRP相关。
BD患者的血浆SFA和MUFA谱不利。与较高血浆hsCRP水平相关的POA可能在BD的发病机制中起作用。
