College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21936, Republic of Korea; Gachon Medical and Convergence Institute, Gachon Gil Medical Center, Incheon 21565, Republic of Korea.
College of Pharmacy, Gachon University, Incheon 21936, Republic of Korea; Lee Gil Ya Cancer and Diabetes Institute, Gachon University, Incheon 21936, Republic of Korea.
Fish Shellfish Immunol. 2018 Aug;79:163-167. doi: 10.1016/j.fsi.2018.05.025. Epub 2018 May 14.
Inflammation markers in zebrafish embryos reflect a toxic response that is common to other animal models and humans. Free fatty acids (FFAs) are known to cause damage in various tissues by inducing inflammation. In this study, we investigated whether a FFA (palmitate) induces inflammation in zebrafish embryos. Nitrous oxide (NO) production and cyclooxygenase-2 (COX-2) mRNA expression were increased in palmitate-treated zebrafish embryos in a dose-dependent manner. mRNA expression of pro-inflammatory cytokines, interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF- α), were also increased. Additionally, the mRNA expression of p65 nuclear factor-kB and I-kB-α were significantly increased after palmitate-treatment. Increased reactive oxygen species (ROS) expression was observed in palmitate-treated zebrafish embryos as well as pericardial edema. Additionally, mRNA expression of pro-inflammatory cytokines were increased in zebrafish liver and pancreas fed with palmitate-contained diet. Taken together, these results indicated that palmitate increases pro-inflammatory mediators in zebrafish embryos, suggesting that zebrafish could be an alternative animal model for inflammatory disease including diabetes.
在斑马鱼胚胎中的炎症标志物反映了一种常见于其他动物模型和人类的毒性反应。游离脂肪酸（FFAs）通过诱导炎症而导致各种组织损伤。在这项研究中，我们研究了 FFA（棕榈酸）是否会引起斑马鱼胚胎的炎症。一氧化氮（NO）的产生和环氧合酶-2（COX-2）mRNA 的表达在棕榈酸处理的斑马鱼胚胎中呈剂量依赖性增加。促炎细胞因子白细胞介素-1β（IL-1β）和肿瘤坏死因子-α（TNF-α）的 mRNA 表达也增加。此外，棕榈酸处理后 p65 核因子-kB 和 I-kB-α 的 mRNA 表达明显增加。在棕榈酸处理的斑马鱼胚胎中观察到活性氧（ROS）的表达增加以及心包水肿。此外，在喂食含有棕榈酸的饮食的斑马鱼肝脏和胰腺中，促炎细胞因子的 mRNA 表达增加。总之，这些结果表明棕榈酸增加了斑马鱼胚胎中的促炎介质，表明斑马鱼可能成为包括糖尿病在内的炎症性疾病的替代动物模型。
