Argraves W S, Deák F, Sparks K J, Kiss I, Goetinck P F
Proc Natl Acad Sci U S A. 1987 Jan;84(2):464-8. doi: 10.1073/pnas.84.2.464.
cDNAs encoding the Mr 54,000 chicken cartilage matrix protein (CMP) were selected from a cartilage cDNA expression library by immunological means. Antibodies elicited against insert-encoded protein purified from one of the clones reacted specifically with chicken CMP in immunoblots of total cartilage extract, providing positive identification of the cDNA clones isolated. The cDNAs detect a 3.4-kilobase transcript that was present in sternal cartilage and in cartilaginous but not in precartilaginous embryonic limb tissues. The cDNAs code for 416 amino acids of the chicken CMP, including its COOH terminus. There are two striking features in the deduced CMP amino acid sequence: first, it contains a region with significant homologies to repeat sequences in the precursor for epidermal growth factor; and second, it is made up of two large homologous repeat sequences. These results provide the first detailed structural information on the CMP and establish it as a developmentally regulated marker of cartilage differentiation.
通过免疫学方法从鸡软骨cDNA表达文库中筛选出编码54,000分子量鸡软骨基质蛋白（CMP）的cDNA。针对从其中一个克隆中纯化出的插入编码蛋白产生的抗体，在总软骨提取物的免疫印迹中与鸡CMP发生特异性反应，从而对分离得到的cDNA克隆进行了阳性鉴定。这些cDNA检测到一个3.4千碱基的转录本，该转录本存在于胸骨软骨和软骨组织中，但不存在于软骨前的胚胎肢体组织中。这些cDNA编码鸡CMP的416个氨基酸，包括其COOH末端。推导的CMP氨基酸序列有两个显著特征：第一，它包含一个与表皮生长因子前体中的重复序列具有显著同源性的区域；第二，它由两个大的同源重复序列组成。这些结果提供了关于CMP的首个详细结构信息，并将其确立为软骨分化的发育调控标志物。
