Faculty of Chemistry, University of Gdansk, Gdansk, Poland.
Electrophoresis. 2019 Jan;40(2):336-342. doi: 10.1002/elps.201800206. Epub 2018 Oct 11.
Systemin (Sys) is an 18-aa plant peptide hormone involved in the regulation of plant's defensive response. Sys is considered as a fast-spreading systemic wound signal. We developed a simple and rapid CE method to monitor the spreading of Sys peptides through tomato plant. A 1,2,3-triazole-linked AZT-systemin conjugate was designed as a model to study the possibility of translocating small cargo molecules 3'-Azido-2',3'-dideoxythymidine by systemin. The Sys peptides (Sys, N-propiolyl Sys, and AZT-systemin conjugate) were injected into the stem and leaves of mature tomato plant. Its transportation throughout the plant tissue was traced by CE. The peptides were clearly visible in the crude tomato exudates and an optimum separation was achieved in 25 mM phosphate "buffer" at pH 2.5 and a voltage of 20 kV using uncoated fused silica capillary. CE analysis showed that Sys peptides are well separated from tomato plant exudates ingredients and are stable in tomato stem and leaf exudates for up to 24 h. CE study revealed that the Sys peptides are effectively spreading throughout tomato stem and leaves and the peptides could be directly detected in the crude plant matrixes. The translocation was strongly inhibited by sodium azide. The results showed that the established CE method can be used to characterize plant peptides spreading under plant physiological conditions.
系统素（Sys）是一种 18 个氨基酸的植物肽激素，参与植物防御反应的调节。Sys 被认为是一种快速传播的系统性伤口信号。我们开发了一种简单快速的 CE 方法来监测 Sys 肽在番茄植物中的扩散。设计了一种 1,2,3-三唑连接的 AZT-系统素缀合物作为模型，以研究系统素转运小 cargo 分子 3'-叠氮-2',3'-二脱氧胸苷的可能性。将 Sys 肽（Sys、N-丙酰基 Sys 和 AZT-系统素缀合物）注射到成熟番茄植株的茎和叶中。通过 CE 追踪其在整个植物组织中的运输。在粗番茄分泌物中可以清楚地看到这些肽，在 pH 2.5 的 25 mM 磷酸盐“缓冲液”中和 20 kV 的电压下，使用未涂覆的熔融石英毛细管可实现最佳分离。CE 分析表明，Sys 肽与番茄植物分泌物成分很好地分离，在番茄茎和叶分泌物中稳定 24 小时以上。CE 研究表明，Sys 肽有效地在番茄茎和叶中扩散，并且可以在粗植物基质中直接检测到这些肽。叠氮化钠强烈抑制了转运。结果表明，所建立的 CE 方法可用于在植物生理条件下表征植物肽的扩散。
