Bai Yu, Chang Cuilan, Du Fuyou, Tan Zhijing, Bai Yu, Liu Huwei
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, Institute of Analytical Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing, P. R. China; Analytical & Characterization, Experiment Management Center, National Institute of Clean-and-Low-Carbon Energy (NICE), Beijing, P. R. China.
Electrophoresis. 2014 Jul;35(14):1984-8. doi: 10.1002/elps.201300434. Epub 2014 May 19.
Systemin is an important group of plant peptide hormones participating in the regulation of plant defensive responses. An improved method, based on dynamic pH junction and capillary electrophoresis-quadrupole time-of-flight mass spectrometry, was developed for online enrichment and sensitive determination of trace systemins in plants. After optimization, the online enrichment factors for six target systemins ranged from 90- to 127-fold. The detection limits reached lower than 0.5 nM, which were comparable with the sensitivity of LC-MS method. Satisfactory quantitative results were obtained in terms of linearity (R(2) ≥ 0.993), dynamic range (3-120 ng/mL), and reproducibility (≤6.7%). For the analysis of real plant samples, a rapid sample preparation method was developed, using two steps of SPE purification with different retention and separation mechanisms. Finally, this method realized the successful detection of tomato systemin and tobacco hydroxyproline-rich systemin I from plant leaves with shorter analysis time.
系统素是一类重要的植物肽激素，参与植物防御反应的调节。基于动态pH连接和毛细管电泳-四极杆飞行时间质谱，开发了一种改进方法，用于在线富集和灵敏测定植物中的痕量系统素。经过优化，六种目标系统素的在线富集倍数在90至127倍之间。检测限低于0.5 nM，与液相色谱-质谱法的灵敏度相当。在线性（R(2)≥0.993）、动态范围（3 - 120 ng/mL）和重现性（≤6.7%）方面获得了令人满意的定量结果。对于实际植物样品的分析，开发了一种快速样品制备方法，采用具有不同保留和分离机制的两步固相萃取净化。最终，该方法实现了从植物叶片中成功检测番茄系统素和烟草富含羟脯氨酸系统素I，且分析时间更短。
