M. H. Chen, Dr. J. E. Mealy, Prof. J. A. Burdick, Department of Bioengineering, University of Pennsylvania, 210 S 33rd St, Philadelphia, PA, 19104, USA.
Dr. J. J. Chung, S. Zaman, E. C. Li, M. F. Arisi, Prof. P. Atluri, Division of Cardiovascular Surgery, Department of Surgery, University of Pennsylvania, Silverstein 6, 3400 Spruce St, Philadelphia, PA, 19104, USA.
Macromol Biosci. 2019 Jan;19(1):e1800248. doi: 10.1002/mabi.201800248. Epub 2018 Sep 27.
Shear-thinning hydrogels are useful for biomedical applications, from 3D bioprinting to injectable biomaterials. Although they have the appropriate properties for injection, it may be advantageous to decouple injectability from the controlled release of encapsulated therapeutics. Toward this, composites of hydrogels and encapsulated microgels are introduced with microgels that are fabricated via microfluidics. The microgel cross-linker controls degradation and entrapped molecule release, and the concentration of microgels alters composite hydrogel rheological properties. For the treatment of myocardial infarction (MI), interleukin-10 (IL-10) is encapsulated in microgels and released from composites. In a rat model of MI, composites with IL-10 reduce macrophage density after 1 week and improve scar thickness, ejection fraction, cardiac output, and the size of vascular structures after 4 weeks when compared to saline injection. Improvements are also observed with the composite without IL-10 over saline, emphasizing the role of injectable hydrogels alone on tissue repair.
剪切稀化水凝胶在生物医学应用中很有用，从 3D 生物打印到可注射生物材料。尽管它们具有可注射的适当特性，但将封装治疗药物的可注射性与控制释放分离可能是有利的。为此，引入了水凝胶和包封微凝胶的复合材料，微凝胶是通过微流控技术制造的。微凝胶交联剂控制降解和包埋分子的释放，而微凝胶的浓度改变复合水凝胶的流变性质。在治疗心肌梗死 (MI) 方面，白细胞介素 10 (IL-10) 被包封在微凝胶中，并从复合材料中释放。在 MI 的大鼠模型中，与盐水注射相比，含有 IL-10 的复合材料在 1 周后降低了巨噬细胞密度，并在 4 周后改善了疤痕厚度、射血分数、心输出量和血管结构的大小。与不含 IL-10 的盐水相比，复合材料也有改善，这强调了可注射水凝胶单独对组织修复的作用。
