海水和淡水虹鳟鱼皮肤胶原蛋白特性的比较。
Comparison of collagen characteristics of sea- and freshwater-rainbow trout skin.
作者信息
Lee Jun Kyu, Kang Sang In, Kim Yong Jung, Kim Min Joo, Heu Min Soo, Choi Byeong Dae, Kim Jin-Soo
机构信息
1Department of Seafood Science and Aquaculture Science and Institute of Marine Industry, Gyeongsang National University, Tongyeong, Gyeongnam, 53064 Korea.
2Department of Food Science and Nutrition, Gyeongsang National University, Jinju, Gyeongnam, 52828 Korea.
出版信息
Food Sci Biotechnol. 2016 Feb 29;25(1):131-136. doi: 10.1007/s10068-016-0020-z. eCollection 2016.
Proximate composition, volatile basic nitrogen content, and concentrations of collagen in skin samples from either sea- (S-RT) or freshwater-rainbow trout (F-RT) were characterized and compared, to assess the effect of the sea or freshwater habitat on these parameters. Results of amino acid composition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, thermal denaturation assay and Fourier transform infrared (FT-IR) spectroscopy of acid-soluble collagens were comparable between the two sample sets. Both acid-soluble collagens from sea- and freshwater-rainbow trout skins contained glycine as the major amino acid and high alanine, proline, and hydroxyproline contents, and was found to be predominantly composed of α1-, α2-, and β-chains. FT-IR spectra of ASCs from S-RT and F-RT skins were quite similar. These findings suggest that different rainbow trout habitats (seawater and freshwater) do not affect amino acid composition and molecular weight properties of ASCs from S-RT and F-RT skins.
对海（S-RT）或淡水虹鳟（F-RT）皮肤样本的近似成分、挥发性盐基氮含量和胶原蛋白浓度进行了表征和比较，以评估海洋或淡水栖息地对这些参数的影响。两个样本组的酸溶性胶原蛋白的氨基酸组成、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳、热变性分析和傅里叶变换红外（FT-IR）光谱结果具有可比性。来自海虹鳟和淡水虹鳟皮肤的酸溶性胶原蛋白均以甘氨酸为主要氨基酸，丙氨酸、脯氨酸和羟脯氨酸含量较高，且主要由α1-、α2-和β链组成。S-RT和F-RT皮肤的ASC的FT-IR光谱非常相似。这些发现表明，不同的虹鳟栖息地（海水和淡水）不会影响S-RT和F-RT皮肤的ASC的氨基酸组成和分子量特性。
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